[Quantitative evaluation of serum IgG subclass levels by an immunoenzyme method]
- PMID: 3238275
- DOI: 10.1016/s0338-4535(88)80077-1
[Quantitative evaluation of serum IgG subclass levels by an immunoenzyme method]
Abstract
We developed a enzyme linked immunosorbent assay (ELISA) for measuring IgG subclasses concentration in serum. For this we used monoclonal antibodies. The specificity of these antibodies was evaluated with a panel of myeloma proteins belonging to the 4 IgG subclasses. The ELISA was sensitive (allowing the detection of subclasses at ng level) and accurate (inter-assay coefficient of variation of 14%). Using the WHO serum 67/97 as reference, we determined the concentration of IgG subclasses in a pool of sera. In addition concentrations were measured in 69 healthy adults to study the distribution of each IgG subclass. A good correlation (r = 0.78) was obtained between the sum of the subclasses measured by ELISA and total IgG measured by immunonephelometry.
Similar articles
-
A quantitative ELISA for antigen-specific IgG subclasses using equivalence dilutions of anti-kappa and anti-subclass specific secondary reagents. Application to the study of the murine immune response against the capsular polysaccharide of Neisseria meningitidis serogroup B.J Immunol Methods. 1996 Apr 19;190(2):221-34. doi: 10.1016/0022-1759(95)00278-2. J Immunol Methods. 1996. PMID: 8621957
-
Dot-immunobinding assay as an accurate and versatile technique for the quantification of human IgG subclasses.J Immunol Methods. 1988 Apr 6;108(1-2):195-203. doi: 10.1016/0022-1759(88)90419-x. J Immunol Methods. 1988. PMID: 3351308
-
Development of a semi-quantitative enzyme-linked immunosorbent assay (ELISA) for detection of human IgG subclass antibodies.J Immunol Methods. 1987 Jan 26;96(1):47-56. doi: 10.1016/0022-1759(87)90366-8. J Immunol Methods. 1987. PMID: 3805734
-
Human IgG subclass measurements in the clinical laboratory.Clin Chem. 1987 Oct;33(10):1707-25. Clin Chem. 1987. PMID: 3311462 Review.
-
Immunoassays for IgG subclasses: quantitative and methodologic considerations.N Engl Reg Allergy Proc. 1988 Jan-Feb;9(1):23-9. doi: 10.2500/108854188778984518. N Engl Reg Allergy Proc. 1988. PMID: 3283516 Review.