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. 2020 Oct;108(7):2961-2970.
doi: 10.1002/jbm.b.34626. Epub 2020 May 9.

High porous electrospun poly(ε-caprolactone)/gelatin/MgO scaffolds preseeded with endometrial stem cells promote tissue regeneration in full-thickness skin wounds: An in vivo study

Shima Ababzadeh  1   2 , Ali Farzin  3   4 , Arash Goodarzi  5 , Roya Karimi  4 , Morteza Sagharjoghi Farahani  2 , Mohsen Eslami Farsani  6 , Kamelia Gharibzad  7 , Maria Zahiri  8 , Jafar Ai  2   4
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High porous electrospun poly(ε-caprolactone)/gelatin/MgO scaffolds preseeded with endometrial stem cells promote tissue regeneration in full-thickness skin wounds: An in vivo study

Shima Ababzadeh et al. J Biomed Mater Res B Appl Biomater. 2020 Oct.

Abstract

In the current study, electrospun poly(ε-caprolactone)-gelatin (PCL-Gel) fibrous scaffolds containing magnesium oxide (MgO) particles and preseeded with human endometrial stem cells (hEnSCs) were developed to use as wound care material in skin tissue engineering applications. Electrospun fibers were fabricated using PCL-Gel (1:1 [wt/wt]) with different concentrations of MgO particles (1, 2, and 4 wt%). The fibrous scaffolds were evaluated regarding their microstructure, mechanical properties, surface wettability, and in vitro and in vivo performances. The full-thickness excisional wound model was used to evaluate the in vivo wound healing ability of the fabricated scaffolds. Our findings confirmed that the wounds covered with PCL-Gel fibrous scaffolds containing 2 wt% MgO and preseeded with hEnSCs have nearly 79% wound closure ability while sterile gauze showed 11% of wound size reduction. Our results can be employed for biomaterials aimed at the healing of full-thickness skin wounds.

Keywords: PCL electrospun scaffold; endometrial stem cells; magnesium oxide; skin tissue regeneration.

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References

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