A novel electrochemical immunosensor for the highly sensitive and selective detection of the depression marker human apolipoprotein A4

Abstract

The fabrication of electrochemical biosensors to directly, rapidly and ultrasensitively detect disease markers in urine or blood samples has become a new and competitive challenge in the field of sensor research. In this paper, a novel electrochemical immunosensor with high selectivity and sensitivity for the detection of the depression marker human apolipoprotein A4 (Apo-A4) was successfully constructed using zeolite imidazole ester metal organic skeleton-nitrogen doped graphene composites (ZIF-8@N-Gr). To this end, because of the higher surface area and biocompatibility, ZIF-8 with abundant biomolecular binding sites provided a good microenvironment for effectively immobilizing antigens. ZIF-8@N-Gr presented a flake structure, as the electrode displayed excellent electrical conductivity, which enhanced the electron transfer and significantly amplified the current signal of the immunosensor. More importantly, these immunosensors are capable of assaying human apolipoprotein A4 (Apo-A4) in 100% serum without suffering from any significant biological interference. Under optimized experimental conditions, the sensor was used for the analysis of whole serum samples and presented a wide linear range from 1.47 × 10^-10 g/mL to 3.00 × 10^-7 g/mL with a low detection limit of 8.33 × 10^-11 g/mL (3σ, n = 15). The satisfactory results of human serum sample analysis indicated that the proposed immunosensor had promising potential in the clinical diagnosis of depression.

Keywords: Apolipoprotein A4; Depression markers; Electrochemical immunosensor.