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. 2020 May 2;14(1):35.
doi: 10.1186/s13065-020-00687-1. eCollection 2020 Dec.

Development and application of a rapid HPLC method for simultaneous determination of hyperoside, isoquercitrin and eleutheroside E in Apocynum venetum L. and Eleutherococcus senticosus

Jie Shen  1 , Kai Yang  2 , Cheng Jiang  3 , Xiao-Qiong Ma  1 , Min-Xia Zheng  1 , Cai-Hua Sun  1
Affiliations

Development and application of a rapid HPLC method for simultaneous determination of hyperoside, isoquercitrin and eleutheroside E in Apocynum venetum L. and Eleutherococcus senticosus

Jie Shen et al. BMC Chem. .

Abstract

Apocynum venetum L. and Eleutherococcus senticosus have been used for hundreds of years to treat hypertension in China. In previous research, there was not a suitable quality control of method for the formulas of Apocynum venetum L. and Eleutherococcus senticosus. It is urgent and essential to develop modern analytical methods for Apocynum venetum L. and Eleutherococcus senticosus to ensure the quality of the formulas. A rapid approach for simultaneous determination of hyperoside, isoquercitrin and eleutheroside E in Apocynum venetum L. and Eleutherococcus senticosus by high-performance liquid chromatography with a diode array detector was described and validated. The full method validation, including the linearity, limits of detection and quantification, precision, repeatability, stability and recovery, was examined. All target components, including isomers of hyperoside and isoquercitrin, were baseline separated in 35 min. The developed method was sensitive, reliable and feasible. With this method, the optimal decoction conditions of Apocynum venetum L. and Eleutherococcus senticosus were selected, and their quality analysis was carried out. Furthermore, an herbal compatibility study of Apocynum venetum L. and Eleutherococcus senticosus based on detecting variations in the content of their active ingredients was performed by the developed HPLC method. It could be an alternative for the quantitative analysis of herbs that contain hyperoside, isoquercitrin or (and) eleutheroside E in the future.

Keywords: Eleutheroside E; Hyperoside; Isoquercitrin; Quality control.

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Conflict of interest statement

Competing interestsAll the authors listed in the manuscript have declared that no competing interests exist.

Figures

Fig. 1
Fig. 1
Chemical structures of hyperoside, isoquercitrin and eleutheroside E
Fig. 2
Fig. 2
HPLC chromatograms of 0.05 mg/mL standard solutions of hyperoside, isoquercitrin and eleutheroside E at 210 nm (blue chromatogram) and 360 nm (red chromatogram) separately. 1. Eleutheroside E, 2. Hyperoside, 3. Isoquercitrin
Fig. 3
Fig. 3
HPLC chromatogram of the electuary of Apocynum venetum L. and Eleutherococcus senticosus formulas under the optimized chromatographic conditions. 1. Eleutheroside E, 2. Hyperoside, 3. Isoquercitrin
Fig. 4
Fig. 4
HPLC chromatograms of the standard solution and sample solution under the optimized chromatographic conditions. A. mixed standard solution, B. decoction of Apocynum venetum L. and Eleutherococcus senticosus, C. granules of Apocynum venetum L. and Eleutherococcus senticosus, D. granules of Eleutherococcus senticosus, E. granules of Apocynum venetum L. 1. eleutheroside E, 2. hyperoside, 3. isoquercitrin
Fig. 5
Fig. 5
The contents of hyperoside, isoquercitrin and eleutheroside E in AV and ES granules, AV granules, and ES granules
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