Prime genome editing in rice and wheat

Qiupeng Lin^#^{1

2}, Yuan Zong^#¹, Chenxiao Xue^#^{1

2}, Shengxing Wang¹, Shuai Jin^{1

2}, Zixu Zhu^{1

2}, Yanpeng Wang^{1

2}, Andrew V Anzalone^{3

4

5}, Aditya Raguram^{3

4

5}, Jordan L Doman^{3

4

5}, David R Liu^{3

4

5}, Caixia Gao^{6

7}

Affiliations

¹ State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, China.
² College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China.
³ Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT, Cambridge, MA, USA.
⁴ Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.
⁵ Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA.
⁶ State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, China. cxgao@genetics.ac.cn.
⁷ College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China. cxgao@genetics.ac.cn.

^# Contributed equally.

PMID: 32393904
DOI: 10.1038/s41587-020-0455-x

Prime genome editing in rice and wheat

Qiupeng Lin et al. Nat Biotechnol. 2020 May.

. 2020 May;38(5):582-585.

doi: 10.1038/s41587-020-0455-x. Epub 2020 Mar 16.

Authors

Affiliations

¹ State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, China.
² College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China.
³ Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT, Cambridge, MA, USA.
⁴ Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.
⁵ Howard Hughes Medical Institute, Harvard University, Cambridge, MA, USA.
⁶ State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, China. cxgao@genetics.ac.cn.
⁷ College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China. cxgao@genetics.ac.cn.

^# Contributed equally.

PMID: 32393904
DOI: 10.1038/s41587-020-0455-x

Abstract

Prime editors, which are CRISPR-Cas9 nickase (H840A)-reverse transcriptase fusions programmed with prime editing guide RNAs (pegRNAs), can edit bases in mammalian cells without donor DNA or double-strand breaks. We adapted prime editors for use in plants through codon, promoter, and editing-condition optimization. The resulting suite of plant prime editors enable point mutations, insertions and deletions in rice and wheat protoplasts. Regenerated prime-edited rice plants were obtained at frequencies of up to 21.8%.

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Comment in

Prime Editing: Game Changer for Modifying Plant Genomes.
Marzec M, Hensel G. Marzec M, et al. Trends Plant Sci. 2020 Aug;25(8):722-724. doi: 10.1016/j.tplants.2020.05.008. Epub 2020 Jun 5. Trends Plant Sci. 2020. PMID: 32513585

References

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1. Svitashev, S. et al. Targeted mutagenesis, precise gene editing, and site-specific gene insertion in maize using Cas9 and guide RNA. Plant Physiol. 169, 931–945 (2015). - DOI - PubMed - PMC

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Prime genome editing in rice and wheat

Affiliations

Prime genome editing in rice and wheat

Authors

Affiliations

Abstract

Comment in

References

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MeSH terms

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Grants and funding

LinkOut - more resources

Full Text Sources

Other Literature Sources

Research Materials