Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2020 Jul;67(4):1485-1491.
doi: 10.1111/tbed.13620. Epub 2020 May 31.

Recent progress on the diagnosis of 2019 Novel Coronavirus

Chun Li  1 , Linzhu Ren  2
Affiliations
Review

Recent progress on the diagnosis of 2019 Novel Coronavirus

Chun Li et al. Transbound Emerg Dis. 2020 Jul.

Erratum in

  • Corrigendum.
    [No authors listed] [No authors listed] Transbound Emerg Dis. 2020 Nov;67(6):3076. doi: 10.1111/tbed.13838. Transbound Emerg Dis. 2020. PMID: 33300309 Free PMC article. No abstract available.

Abstract

Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has become a global pandemic. Therefore, convenient, timely and accurate detection of SARS-CoV-2 is urgently needed. Here, we review the types, characteristics and shortcomings of various detection methods, as well as perspectives for the SARS-CoV-2 diagnosis. Clinically, nucleic acid-based methods are sensitive but prone to false-positive. The antibody-based method has slightly lower sensitivity but higher accuracy. Therefore, it is suggested to combine the two methods to improve the detection accuracy of COVID-19.

Keywords: Corona Virus Disease 2019 (COVID-19); antibody; clustered regularly interspaced short palindromic repeats (CRISPR); detection; real-time RT-PCR; reverse transcription loop-mediated isothermal amplification (RT-LAMP); severe acute respiratory syndrome coronavirus 2 (2019 Novel Coronavirus SARS-CoV-2).

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

References

    1. Adams, E. R. , Ainsworth, M. , Anand, R. , Andersson, M. I. , Auckland, K. , Baillie, J. K. , … Whitehouse, J. (2020). Antibody testing for COVID‐19: A report from the National COVID Scientific Advisory Panel. medRxiv, 2020.04.15.20066407. 10.1101/2020.04.15.20066407 - DOI - PMC - PubMed
    1. Association, S. E. G. f. C. o. t. E. o. N. C. P. o. t. C. P. M (2020). An update on the epidemiological characteristics of novel coronavirus pneumonia(COVID‐19). Zhonghua Liu Xing Bing Xue Za Zhi, 41, 139–144. - PubMed
    1. Baek, Y. H. , Um, J. , Antigua, K. J. C. , Park, J. H. , Kim, Y. , Oh, S. , … Song, M. S. (2020). Development of a reverse transcription‐loop‐mediated isothermal amplification as a rapid early‐detection method for novel SARS‐CoV‐2. Emerging Microbes & Infections, 9, 1–31. 10.1080/22221751.2020.1756698 - DOI - PMC - PubMed
    1. Broughton, J. P. , Deng, W. , Fasching, C. L. , Singh, J. , Chiu, C. Y. , & Chen, J. S. (2020). A protocol for rapid detection of the 2019 novel coronavirus SARS‐CoV‐2 using CRISPR diagnostics: SARS‐CoV‐2 DETECTR. Available: https://mammoth.bio/wp‐content/uploads/2020/02/A‐protocol‐for‐rapid‐dete...
    1. Cai, X. , Chen, J. , Hu, J. , Long, Q. , Deng, H. , Fan, K. , & Wang, D. (2020). A peptide‐based magnetic chemiluminescence enzyme immunoassay for serological diagnosis of corona virus disease 2019 (COVID‐19). medRxiv, 2020.2002.2022.20026617. 10.1101/2020.02.22.20026617 - DOI - PMC - PubMed

MeSH terms