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. 2020 Mar;14(2):294-298.
doi: 10.1055/s-0040-1709944. Epub 2020 May 12.

Anti-Inflammatory Effect of Musa acuminata Stem

Maharani Laillyza Apriasari  1 , Selviana Rizky Pramitha  1 , Dewi Puspitasari  2 , Diah Savitri Ernawati  3
Affiliations

Anti-Inflammatory Effect of Musa acuminata Stem

Maharani Laillyza Apriasari et al. Eur J Dent. 2020 Mar.

Abstract

Objective: This study was designed to assess the anti-inflammatory effect of Musa acuminata through the expression of tumor necrosis factor-α (TNF-α) and nuclear factor kappa β (NF-κB) after 3 days of application of Musa acuminata stem extract (MASE) gel on oral mucosal wound.

Materials and methods: An experimental study with post-test only control group design was conducted. Twenty male Rattus norvegicus (Wistar) were injured on their left buccal mucosa and treated three times a day with MASE gel of varying concentrations: 0% (as control), MASE 25%, MASE 37.5%, and MASE 50%. On day 3, a biopsy was performed on each mucosal wound for later immunohistochemical analysis for the expressions of TNF-α and NF-κB.

Results: The highest expression of TNF-α was observed in the control group (13.20 ± 1.79), while the lowest was in the treatment group using 50% MASE (6.40 ± 1.14). Meanwhile the comparison between treatment groups did not highlight any significant difference (p > 0.05). The highest expression of NF-κB was observed in the control group (13.20 ± 1.30), whereas the lowest was in the treatment group using MASE 50% (6.40 ± 1.14). NF-κB was significantly lower in the treatment group using MASE 50% when compared with other treatment groups (p < 0.05).

Conclusion: Application of MASE on mucosal wound reduces the expression of TNF-α and NF-κB at all concentrations. The anti-inflammatory effect of MASE 50% was the strongest one.

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Conflict of interest statement

None declared.

Figures

Fig. 1
Fig. 1
Macrophage’s expression of tumor necrosis factor-α(TNF-α; black arrow) on control group ( A ), Musa acuminata stem extract (MASE) concentrations of 25% ( B ), MASE concentrations of 37.5% (C), and MASE concentrations of 50% ( D ).
Fig. 2
Fig. 2
Macrophage expression of nuclear factor kappa β(NF-κB; black arrow) in control group ( A ), Musa acuminata stem extract (MASE) concentrations of 25% ( B ), MASE concentrations of 37.5% (C), and MASE concentrations of 50% ( D ).
See this image and copyright information in PMC

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