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. 2020 Aug;27(22):27762-27772.
doi: 10.1007/s11356-020-09006-6. Epub 2020 May 12.

Isolation and characterization of biosurfactant-producing Serratia marcescens ZCF25 from oil sludge and application to bioremediation

Yi Huang #  1 , Hanghai Zhou #  1 , Gang Zheng  2 , Yanhong Li  3   4 , Qinglin Xie  3   4 , Shaohong You  3   4 , Chunfang Zhang  5   6
Affiliations

Isolation and characterization of biosurfactant-producing Serratia marcescens ZCF25 from oil sludge and application to bioremediation

Yi Huang et al. Environ Sci Pollut Res Int. 2020 Aug.

Abstract

A biosurfactant (BS) is a surface-active metabolite that is secreted by microbial metabolism, and can be used as a substitute for chemically synthesized surfactants. The first and most critical step to the successful application of BSs is to isolate bacterial strains with strong BS-producing capabilities. In this study, a BS-producing Serratia marcescens ZCF25 was isolated from the sludge of an oil tanker. Through polyphasic characterization using Fourier-transform infrared spectroscopy, thin layer chromatography, and gas chromatography-mass spectrometry, the produced BS was classified as a lipopeptide; it can decrease the water surface tension from 72.0 to 29.50 mN m-1 and has a critical micelle concentration of 220 mg/L. The BS showed a high tolerance over a wide range of pH (2-12), temperature (50-100 °C), and salinity (10-100 g/L). Furthermore, the inoculation of S. marcescens ZCF25 with fracturing flowback fluids could significantly (P < 0.05) reduce the chemical oxygen demand, concentration of alkanes, and concentration of polycyclic aromatic hydrocarbons, with removal efficiencies of 48.9%, 65.57%, and 64%, respectively. This is the first study on the application of BS-producing S. marcescens to treat fracturing flowback fluids. S. marcescens ZCF25 is a promising candidate for use in various industrial and bioremediation applications. Graphical abstract.

Keywords: Bioremediation; Biosurfactant; Fracturing flowback fluids; Lipopeptide; Pseudomonas; Serratia marcescens.

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