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Review
. 2020 May;4(5):e1900247.
doi: 10.1002/adbi.201900247. Epub 2020 Mar 2.

Synthetic mRNA-Based Systems in Mammalian Cells

Hirohisa Ohno  1 , Sae Akamine  1 , Hirohide Saito  1
Affiliations
Review

Synthetic mRNA-Based Systems in Mammalian Cells

Hirohisa Ohno et al. Adv Biosyst. 2020 May.

Abstract

Living organisms are programmed to perform multiple functions by sensing intra- and extra-cellular environments and by controlling gene expressions. Synthetic biologists aim to program cells by mimicking, designing, and constructing genetic circuits. Synthetic mRNA-based genetic switches and circuits have attracted attention for future therapeutic applications because of their safety and functional diversity. Here, the mRNA-based switches and circuits that detect specific microRNAs or proteins expressed in a target cell to control transgene expression and cell fate are reviewed. Future perspectives of artificial RNA systems for cell engineering will also be addressed.

Keywords: RNA switch; RNA-binding proteins; gene circuits; mRNA; microRNA; synthetic biology; translational regulation.

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References

    1. J. A. Bernal, J. Cardiovasc. Transl. Res. 2013, 6, 956.
    1. B. Vallazza, S. Petri, M. A. Poleganov, F. Eberle, A. N. Kuhn, U. Sahin, Wiley Interdiscip. Rev.: RNA 2015, 6, 471.
    1. S. L. Ameres, P. D. Zamore, Nat. Rev. Mol. Cell Biol. 2013, 14, 475.
    1. L. F. R. Gebert, I. J. MacRae, Nat. Rev. Mol. Cell Biol. 2019, 20, 21.
    1. P. Landgraf, M. Rusu, R. Sheridan, A. Sewer, N. Iovino, A. Aravin, S. Pfeffer, A. Rice, A. O. Kamphorst, M. Landthaler, C. Lin, N. D. Socci, L. Hermida, V. Fulci, S. Chiaretti, R. Foa, J. Schliwka, U. Fuchs, A. Novosel, R. U. Muller, B. Schermer, U. Bissels, J. Inman, Q. Phan, M. Chien, D. B. Weir, R. Choksi, G. De Vita, D. Frezzetti, H. I. Trompeter, V. Hornung, G. Teng, G. Hartmann, M. Palkovits, R. Di Lauro, P. Wernet, G. Macino, C. E. Rogler, J. W. Nagle, J. Ju, F. N. Papavasiliou, T. Benzing, P. Lichter, W. Tam, M. J. Brownstein, A. Bosio, A. Borkhardt, J. J. Russo, C. Sander, M. Zavolan, T. Tuschl, Cell 2007, 129, 1401.

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