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. 2020 Jul:128:104433.
doi: 10.1016/j.jcv.2020.104433. Epub 2020 May 13.

Real-time PCR-based SARS-CoV-2 detection in Canadian laboratories

Jason J LeBlanc  1 , Jonathan B Gubbay  2 , Yan Li  3 , Robert Needle  4 , Sandra Radons Arneson  3 , Dionne Marcino  3 , Hugues Charest  5 , Guillaume Desnoyers  6 , Kerry Dust  7 , Ramzi Fattouh  8 , Richard Garceau  6 , Gregory German  9 , Todd F Hatchette  10 , Robert A Kozak  11 , Mel Krajden  12 , Theodore Kuschak  3 , Amanda L S Lang  13 , Paul Levett  12 , Tony Mazzulli  14 , Ryan McDonald  13 , Samira Mubareka  11 , Natalie Prystajecky  12 , Candy Rutherford  15 , Marek Smieja  15 , Yang Yu  4 , George Zahariadis  4 , Nathan Zelyas  16 , Nathalie Bastien  3 ; COVID-19 Pandemic Diagnostics Investigation Team of the Canadian Public Health Laboratory Network (CPHLN) Respiratory Virus Working Group
Affiliations

Real-time PCR-based SARS-CoV-2 detection in Canadian laboratories

Jason J LeBlanc et al. J Clin Virol. 2020 Jul.

Abstract

With emergence of pandemic COVID-19, rapid and accurate diagnostic testing is essential. This study compared laboratory-developed tests (LDTs) used for the detection of SARS-CoV-2 in Canadian hospital and public health laboratories, and some commercially available real-time RT-PCR assays. Overall, analytical sensitivities were equivalent between LDTs and most commercially available methods.

Keywords: COVID-19; Coronavirus; Molecular; NAAT; PCR; SARS-CoV-2.

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Conflict of interest statement

Declaration of Competing Interest The authors have no conflicts to declare

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