Cytoplasmic diversity of potato relatives preserved at Plant Breeding and Acclimatization Institute in Poland

Abstract

Among different types of potato cytoplasmic genomes, some are associated with male sterility or affect agronomic traits. The goal of this study was to analyze types of chloroplast and mitochondrial genomes of selected potato relatives originating from collection of the Institute of Plant Industry, Saint Petersburg, Russia, and preserved in Poland. Using chloroplast and mitochondrial markers the cytoplasm types were determined for 401 genotypes belonging to 43 seed accessions of 28 Solanum species. Among characterized genotypes, 201 (50.1%), 156 (38.9%) and 44 (11%) had cytoplasm types W, D, M, respectively. No accessions with the T, P or A cytoplasm were found. Within cytoplasm W, genotypes with the subtypes: W/α and W/β were identified, but not with W/γ. In S. famatinae, we detected unusual product of the T marker with 65 bp insertion earlier seen exclusively in S. vernei. Among the genotypes of S. leptophyes, two profiles of the ALM_4/ALM_5 marker were observed. S. famatinae and S. vernei come from Argentina, provinces Catamarca and Tucumán. Possibly the insertion in marker T occurred independently in two species, or the accessions were misidentified. Segregation of the ALM_4/ALM_5 marker within S. leptophyes indicates that potato seed accessions are heterogeneous not only due to nuclear DNA polymorphisms but have diversified cytoplasm, too. Our findings are important for exploitation of the tested material in potato breeding. Male-fertile cytoplasm types give a chance of avoiding fertility problems and widening the range of crosses in future generations of breeding materials.

Keywords: Chloroplast; Cytoplasm types; Mitochondrion; Solanum; Wild potato species.

Fig. 1

Different amplification of T marker in multiplex PCR after BamHI digestion within S. famatinae (RUS001:4304; POL003:333139) individuals (samples 4–7). Samples 1–3 S. dolichostigma (RUS001:7613; POL003:333115); 8–11 S. fendleri (RUS001:5751; POL003:333112); 12–15 S. fendleri (RUS001:5747; POL003:333143); 16–18 S. fendleri (RUS001:5671; POL003:333110). Standards: wild species S. michoacanum (W), cv. Early Rose (T), breeding line PW 363 (D), cultivated species S. phureja (P) and cv. Maris Piper (A). T marker is marked with an arrow