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Review
. 2020 Oct;38(10):1047-1051.
doi: 10.1016/j.tibtech.2020.04.008. Epub 2020 May 11.

Minicircle DNA: The Future for DNA-Based Vectors?

Ana Margarida Almeida  1 , João António Queiroz  1 , Fani Sousa  1 , Ângela Sousa  2
Affiliations
Review

Minicircle DNA: The Future for DNA-Based Vectors?

Ana Margarida Almeida et al. Trends Biotechnol. 2020 Oct.

Abstract

Minicircle DNA (mcDNA) is a smaller and safer version of non-viral DNA vectors that results from a cutting-edge in vivo recombination process to excise prokaryotic sequences from plasmid DNA (pDNA). Considering the molecule's potential and increasing interest as a non-viral DNA-based therapeutic, biomanufacturing methodologies need to be improved, especially in downstream processing.

Keywords: downstream processing; gene delivery; minicircle DNA biomanufacturing; plasmid DNA; upstream processing.

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