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. 2020:2144:1-6.
doi: 10.1007/978-1-0716-0592-9_1.

Measuring the Replicative Lifespan of Saccharomyces cerevisiae Using the HYAA Microfluidic Platform

Ruofan Yu  1   2 , Myeong Chan Jo  3 , Weiwei Dang  4   5
Affiliations

Measuring the Replicative Lifespan of Saccharomyces cerevisiae Using the HYAA Microfluidic Platform

Ruofan Yu et al. Methods Mol Biol. 2020.

Abstract

The replicative aging of the budding yeast, Saccharomyces cerevisiae, has been a useful model for dissecting the molecular mechanisms of the aging process. Traditionally, the replicative lifespan (RLS) is measured by manually dissecting mother cells from daughter cells, which is a very tedious process. Since 2012, several microfluidic systems have been developed to automate the dissection process, significantly accelerating RLS determination. Here, we describe a detailed protocol of RLS measurement using a ommercially available microfluidic system based on the HYAA chip design, which enables data collection of up to 8000 cells in a single experiment.

Keywords: Budding yeast; Microfluidics; Replicative aging; Replicative lifespan.

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Figures

Figure 1:
Figure 1:
Microfluidic platform for yeast RLS measurement. Up: The platform consists of pump system and live cell imaging system connected with environmental chamber control unit.
Figure 2:
Figure 2:
Schematic design of HYAA microfluidic chip.
See this image and copyright information in PMC

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