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. 2020:2146:171-184.
doi: 10.1007/978-1-0716-0603-2_13.

Laser Microdissection as a Useful Tool to Study Gene Expression in Plant and Fungal Partners in AM Symbiosis

Raffaella Balestrini  1 , Valentina Fiorilli  2
Affiliations

Laser Microdissection as a Useful Tool to Study Gene Expression in Plant and Fungal Partners in AM Symbiosis

Raffaella Balestrini et al. Methods Mol Biol. 2020.

Abstract

Laser microdissection (LMD) technology has been widely applied to plant tissues, offering novel information on the role of different cell-type populations during plant-microbe interactions. In this chapter, protocols to apply the LMD approach to study plant and fungal transcript profiles in different cell-type populations from arbuscular mycorrhizal (AM) roots are described in detail, starting from the biological material preparation to gene expression analyses by RT-PCR and RT-qPCR.

Keywords: AM fungi; AM symbiosis; Cell specificity; Gene expression; Laser microdissection.

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