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. 2020 Aug 24;59(35):15152-15156.
doi: 10.1002/anie.202004142. Epub 2020 Jun 8.

Real-Time In Vivo Detection of Cellular Senescence through the Controlled Release of the NIR Fluorescent Dye Nile Blue

Beatriz Lozano-Torres  1   2   3   4 , Juan F Blandez  1   2   4 , Irene Galiana  1   2 , Alba García-Fernández  3 , María Alfonso  1 , María D Marcos  1   2   3   4 , Mar Orzáez  2   5 , Félix Sancenón  1   2   3   4 , Ramón Martínez-Máñez  1   2   3   4
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Free article

Real-Time In Vivo Detection of Cellular Senescence through the Controlled Release of the NIR Fluorescent Dye Nile Blue

Beatriz Lozano-Torres et al. Angew Chem Int Ed Engl. .
Free article

Abstract

In vivo detection of cellular senescence is accomplished by using mesoporous silica nanoparticles loaded with the NIR-FDA approved Nile blue (NB) dye and capped with a galactohexasaccharide (S3). NB emission at 672 nm is highly quenched inside S3, yet a remarkable emission enhancement is observed upon cap hydrolysis in the presence of β-galactosidase and dye release. The efficacy of the probe to detect cellular senescence is tested in vitro in melanoma SK-Mel-103 and breast cancer 4T1 cells and in vivo in palbociclib-treated BALB/cByJ mice bearing breast cancer tumor.

Keywords: NIR probes; Nile blue; in vivo detection; mesoporous nanoparticles; senescence.

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References

    1. S. He, N. E. Sharpless, Cell 2017, 169, 1000-1011.
    1. None
    1. B. Lozano-Torres, A. Estepa-Fernández, M. Rovira, M. Orzáez, M. Serrano, R. Martínez-Máñez, F. Sancenón, Nat. Rev. Chem. 2019, 3, 426-441;
    1. D. Muñoz-Espín, M. Serrano, Nat. Rev. Mol. Cell Biol. 2014, 15, 482-496.
    1. A. Hernandez-Segura, J. Nehme, M. Demaria, Trends Cell Biol. 2018, 28, 436-453.

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