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Comment
. 2020 Jul;41(7):429-431.
doi: 10.1016/j.tips.2020.04.009. Epub 2020 May 13.

Rewired Cas9s with Minimal Sequence Constraints

Sébastien Levesque  1 , Daniel Agudelo  1 , Yannick Doyon  2
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Comment

Rewired Cas9s with Minimal Sequence Constraints

Sébastien Levesque et al. Trends Pharmacol Sci. 2020 Jul.

Abstract

The genome editing toolkit is ever expanding. Although CRISPR-Cas systems can target virtually any gene, single-nucleotide resolution is yet to be achieved. Walton and colleagues engineered nucleases and base editors compatible with every protospacer adjacent motif (PAM) to achieve high-precision targeting. Their findings revealed the striking plasticity of Cas9.

Keywords: CRISPR-Cas9; genome editing; protospacer adjacent motifs.

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