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. 2020 Apr;58(2):135-145.
doi: 10.3347/kjp.2020.58.2.135. Epub 2020 Apr 30.

In Vitro Effect of Methanolic Extract of Argemone mexicana against Trichomonas vaginalis

Joel Horacio Elizondo-Luevano  1 , Julia Verde-Star  1 , Azucena González-Horta  1 , Rocío Castro-Ríos  2 , Magda Elizabeth Hernández-García  3 , Abelardo Chávez-Montes  1
Affiliations

In Vitro Effect of Methanolic Extract of Argemone mexicana against Trichomonas vaginalis

Joel Horacio Elizondo-Luevano et al. Korean J Parasitol. 2020 Apr.

Abstract

Infections caused by Trichomonas vaginalis in humans are one of the main public health problems caused by sexually transmitted diseases. Objective of this study was to evaluate potential biological activity of the medicinal plant Argemone mexicana (Mexican poppy) on T. vaginalis. Methanolic extracts of the stems and leaves of A. mexicana, and different fractions were prepared with solvents of different polarities. The extracts and functional groups were detected containing sterols, triterpenes, quinones, flavonoids and, alkaloids. Extracts from both the stems and leaves of A. mexicana inhibited the growth of T. vaginalis with half-maximal inhibitory concentration value of 70.6 and 67.2 μg/ml, respectively. In the active fractions, the most abundant compounds were berberine and jatrorrhizine, with presumed antiparasitic activity.

Keywords: Argemone mexicana; Trichomonas vaginalis; alkaloids; trichomoniasis.

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Conflict of interest statement

CONFLICTS OF INTEREST

The authors declare that there is no conflict of interest regarding the publication of this paper.

Figures

Fig. 1
Fig. 1
HPLC-DAD chromatograms for AmexS (A), AmexL (B) crude extracts and berberine (C) solutions (15 mg/ml).
Fig. 2
Fig. 2
Growth kinetics of Trichomonas vaginalis in TYI-S-33 medium. Each point is mean of 3 independent counts.
Fig. 3
Fig. 3
Direct infusion-electrospray ionization-ion-trap MS2 full scan product. Ion mass spectra of [M]+ ions of (A) berberine (m/z 336) and (B) jatrorrhizine (m/z 338) obtained from the AmexL3 fraction.
Fig. 4
Fig. 4
Hemolytic cytotoxicity of AmexS and AmexL extracts. The results in the columns are shown as the percentage of hemolysis. Values are shown as mean±SD. All assays were performed in triplicate.
See this image and copyright information in PMC

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