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. 2020 Apr 28:2020:9238797.
doi: 10.1155/2020/9238797. eCollection 2020.

Huzhang Tongfeng Granule Improves Monosodium Urate-Induced Inflammation of Gouty Arthritis Rat Model by Downregulation of Cyr61 and Related Cytokines

Mi Zhou  1   2 , Kan Ze  1   2 , Yifei Wang  1   2 , Xin Li  1   2 , Liang Hua  1   2 , Yi Lu  1   2 , Xi Chen  1   2 , Xiaojie Ding  1   2 , Siting Chen  1   2 , Yi Ru  1   2 , Ming Zhang  1   2 , Bin Li  1   2
Affiliations

Huzhang Tongfeng Granule Improves Monosodium Urate-Induced Inflammation of Gouty Arthritis Rat Model by Downregulation of Cyr61 and Related Cytokines

Mi Zhou et al. Evid Based Complement Alternat Med. .

Abstract

Objective: Gouty arthritis (GA) is a noninfectious inflammatory disease characterized by self-limited and severe pain. Huzhang Tongfeng granule is one of the most effective traditional Chinese medicines in the treatment of acute GA. However, its effects on the inflammatory factors in the process of acute gout inflammation remain unknown. In the present study, we aimed to evaluate the effect of Huzhang Tongfeng granule on the expressions of Cyr61 and related inflammatory factors in both experimental gout models in vivo and in vitro.

Methods: Huzhang Tongfeng granule was provided by the pharmaceutical preparation room of Yueyang Hospital of Integrated Traditional Chinese and Western Medicine. The expressions of Cyr61, IL-1β, TNF-α, and IL-6 in monosodium urate- (MSU-) induced rat models and fibroblast-like synoviocytes (FLSs) were determined by RT-PCR, Western blotting analysis, ELISA, immunohistochemistry, and hematoxylin and eosin staining.

Results: Huzhang Tongfeng granule could downregulate the expressions of IL-1β, TNF-α, and IL-6 to some extent by inhibiting the expression of Cyr61.

Conclusions: Collectively, our findings indicated that Cyr61 was highly expressed in rat models of gout. By inhibiting the expression of Cyr61, Huzhang Tongfeng granule could partially attenuate the inflammation induced by MSU crystal.

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Conflict of interest statement

The authors declare that they have no conflicts of interest regarding the publication of this paper.

Figures

Figure 1
Figure 1
(a) The pictures of arthritis from the blank, model, Huzhang-low, and Huzhang-high groups after 72 h. (b) The ratio of the right foot to the left foot (normal control) from 0 h to 72 h. (c) H&E staining of synovial tissue from each group (200x). ∗P < 0.05 vs. blank.
Figure 2
Figure 2
(a) Relative expressions of Cyr61, IL-1β, TNF-α, and IL-6 at the mRNA level in the blank, model, Huzhang-low, and Huzhang-high groups by PCR. (b) The concentrations of IL-1β, TNF-α, and IL-6 were verified by ELISA in the same groups. (c) The protein level of Cyr61 was verified by Western blotting analysis in the rat synovial tissue from the same groups. (d) The protein level in different groups was expressed as a ratio to GAPDH. ∗P < 0.05 vs. blank, #P < 0.05 vs. model.
Figure 3
Figure 3
The IHC pictures of arthritis from the blank, model, Huzhang-low, and Huzhang-high groups (200x).
Figure 4
Figure 4
(a) The pictures of arthritis from the blank, model, Huzhang, colchicines, and shRNA groups after 72 h. Colchicine was used as a positive control. (b) The ratio of the right foot to the left foot (normal control) from 0 h to 72 h. (c) H&E staining of synovial tissue from each group (200x). (d) Relative expressions of Cyr61, IL-1β, TNF-α, and IL-6 at the mRNA level in the same group by PCR. (e) The concentrations of IL-1β, TNF-α, and IL-6 were verified by ELISA in the same groups. ∗P < 0.05 vs. blank, #P < 0.05 vs. model.
Figure 5
Figure 5
(a) Relative expressions of IL-1β, TNF-α, and IL-6 at the mRNA level in the blank, MSU-induced, and Huzhang groups with or without shRNA interference. (b) Protein levels of IL-1β, TNF-α, and IL-6 from the same groups by ELISA. (c) Protein levels of IL-1β, TNF-α, and IL-6 in the same groups. (d) The protein level in the same groups was expressed as a ratio to GAPDH. ∗P < 0.05 vs. blank, #P < 0.05 vs. model.
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