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. 2020 May 1;5(18):10395-10400.
doi: 10.1021/acsomega.0c00283. eCollection 2020 May 12.

Benlysta-Loaded Sodium Alginate Hydrogel and Its Selective Functions in Promoting Skin Cell Growth and Inhibiting Inflammation

Xujia Wang  1 , Shuaimeng Guan  2 , Kun Zhang  2 , Jingan Li  3
Affiliations

Benlysta-Loaded Sodium Alginate Hydrogel and Its Selective Functions in Promoting Skin Cell Growth and Inhibiting Inflammation

Xujia Wang et al. ACS Omega. .

Abstract

Benlysta is a new drug approved by the US Food and Drug Administration (US FDA) in 2019 for the treatment of systemic lupus erythematosus. In this study, we loaded the benlysta in the traditional sodium alginate (SA) hydrogel to investigate the potential application of the drug-loaded hydrogel for skin dressing or hypodermic drug. Live/dead staining images and the CCK-8 results showed that the benlysta-loaded hydrogel could promote the growth of human epidermal cells (HaCat), fibroblasts (L929), and endothelial cells while inhibiting the aggregation of inflammatory cells (macrophages). In addition, the hydrogel degradation and drug release are slow and controllable, and the gel time of drug-loaded hydrogel can be adjusted by adding sodium alginate ratios according to the requirement. In summary, we prepared a time-dependent drug-loaded hydrogel for potential application in the treatment of skin injury that may be caused by other diseases.

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Conflict of interest statement

The authors declare no competing financial interest.

Figures

Figure 1
Figure 1
Pictorial diagram of the gelation of the benlysta-loaded SA hydrogel.
Figure 2
Figure 2
Gelation time of the benlysta-loaded hydrogels with 1 and 2% SA ratios (**p < 0.01, mean ± SD, n = 3).
Figure 3
Figure 3
Water content of the benlysta-loaded hydrogels with 1 and 2% SA ratios (mean ± SD, n = 3).
Figure 4
Figure 4
Swelling rate of the benlysta-loaded hydrogel (mean ± SD, n = 3).
Figure 5
Figure 5
Benlysta release from the SA hydrogel (mean ± SD, n = 3).
Figure 6
Figure 6
Degradation detection of the benlysta-loaded SA hydrogel (mean ± SD, n = 3).
Figure 7
Figure 7
(A) Acridine orange and ethidium bromide (AO/EB) staining images and (B) CCK-8 detection of macrophages in the benlysta-loaded SA hydrogel group and controls (***p < 0.001 compared with other samples, mean ± SD, n = 3).
Figure 8
Figure 8
(A) AO/EB staining images and (B) CCK-8 detection of L929 cell line in the benlysta-loaded SA hydrogel group and controls (*p < 0.05 compared with other samples, &p < 0.05 compared with the control group, mean ± SD, n = 3).
Figure 9
Figure 9
(A) AO/EB staining images and (B) CCK-8 detection of HaCat cell line in the benlysta-loaded SA hydrogel group and controls (*p < 0.05 compared with other samples, &p < 0.05 compared with the control group, mean ± SD, n = 3).
Figure 10
Figure 10
(A) Phalloidin (cytoskeleton) and 4,6-diamino-2-phenyl indole (DAPI) (nucleus) staining images and (B) counting results of endothelial cells in the benlysta-loaded SA hydrogel group and controls (*p < 0.05 compared with other samples, &p < 0.05 compared with the control group, mean ± SD, n = 3).
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