Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Oct;34(10):e4902.
doi: 10.1002/bmc.4902. Epub 2020 Jun 11.

Rapid and cost-effective LC-MS/MS method for determination of hydroxycitric acid in plasma: Application in the determination of pharmacokinetics in commercial Garcinia preparations

Priyadeep Bhutani  1   2 , Rekha U  3 , Shivakumar H N  3 , Prabhakar K Ranjanna  2 , Atish T Paul  1
Affiliations

Rapid and cost-effective LC-MS/MS method for determination of hydroxycitric acid in plasma: Application in the determination of pharmacokinetics in commercial Garcinia preparations

Priyadeep Bhutani et al. Biomed Chromatogr. 2020 Oct.

Abstract

Garcinia cambogia is one of the most commonly used anti-obesity dietary supplements, and hydroxycitric acid (HCA) is a major constituent in the commercial preparations of Garcinia. High doses of HCA are often consumed without much awareness of its pharmacokinetic and toxicokinetic parameters, and therefore, a complete understanding of its effects is lacking. The first step in understanding these parameters is the availability of a reliable bioanalytical method. Here, we present the first report on a UPLC-MS/MS method for analysis of HCA in rat plasma after a simplified and cost-effective protein precipitation. Chromatographic separation of the analytes in the supernatant was achieved using hydrophilic interaction liquid chromatography, where mass parameters were optimized and a rapid 5-min quantitative assay was developed. The method was highly sensitive, accurate, precise and linear in the concentration range of 10.5-5000 ng/mL (validated according to the United States Food and Drug Administration guidelines). Further, the method was successfully used to describe the pharmacokinetic profile of HCA in rat plasma after the administration of pure HCA and commercial Garcinia preparations.

Keywords: Garcinia; LC-MS/MS; bioanalytical method validation; dietary supplement; hydroxycitric acid.

PubMed Disclaimer

References

REFERENCES

    1. Bhutani, P., Murugesan, S., Kumar, A., Subramanian, M., & Prabhakar, K. R. (2018). Offline derivatization LC-MS/MS method for simultaneous estimation of vanillin and vanillic acid in guinea pig plasma. Bioanalysis, 10(3), 131-142. https://doi.org/10.4155/bio-2017-0213
    1. Cho, H. K., Han, Y. S., & Park, J. M. (2019). Ocular complications of Garcinia cambogia extract diet pills: Case report. European Journal of Ophthalmology, 519-547. https://doi.org/10.1177/1120672119872364
    1. Chuah, L. O., Ho, W. Y., Beh, B. K., & Yeap, S. K. (2013). Updates on Antiobesity Effect of Garcinia Origin (−)-HCA. Evidence Based Complementary and Alternative Medicine, 2013, 751658, 1-17. https://doi.org/10.1155/2013/751658
    1. Crescioli, G., Lombardi, N., Bettiol, A., Marconi, E., Risaliti, F., Bertoni, M., … Vannacci, A. (2018). Acute liver injury following Garcinia cambogia weight-loss supplementation: case series and literature review. Internal Emergency Medicine, 13(6), 857-872. https://doi.org/10.1007/s11739-018-1880-4
    1. Davies, B., & Morris, T. (1993). Physiological parameters in laboratory animals and humans. Pharmaceutical Research, 10(7), 1093-1095. https://doi.org/10.1023/A:1018943613122

LinkOut - more resources