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. 2020 Sep;117(9):2771-2780.
doi: 10.1002/bit.27438. Epub 2020 Jun 3.

Microbial production of 4-amino-1-butanol, a four-carbon amino alcohol

Cindy Pricilia Surya Prabowo  1   2 , Jae Ho Shin  1   2 , Jae Sung Cho  1   2 , Tong Un Chae  1   2 , Sang Yup Lee  1   2   3
Affiliations

Microbial production of 4-amino-1-butanol, a four-carbon amino alcohol

Cindy Pricilia Surya Prabowo et al. Biotechnol Bioeng. 2020 Sep.

Abstract

4-Amino-1-butanol (4AB) serves as an important intermediate compound for drugs and a precursor of biodegradable polymers used for gene delivery. Here, we report for the first time the fermentative production of 4AB from glucose by metabolically engineered Corynebacterium glutamicum harboring a newly designed pathway comprising a putrescine (PUT) aminotransferase (encoded by ygjG) and an aldehyde dehydrogenase (encoded by yqhD) from Escherichia coli, which convert PUT to 4AB. Application of several metabolic engineering strategies such as fine-tuning the expression levels of ygjG and yqhD, eliminating competing pathways, and optimizing culture condition further improved 4AB production. Fed-batch culture of the final metabolically engineered C. glutamicum strain produced 24.7 g/L of 4AB. The strategies reported here should be useful for the microbial production of primary amino alcohols from renewable resources.

Keywords: 4-amino-1-butanol; Corynebacterium glutamicum; amino alcohol; metabolic engineering; putrescine.

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References

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