Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Feb;199(2):703-711.
doi: 10.1007/s12011-020-02176-x. Epub 2020 May 21.

Mitophagy Induced by Mitochondrial Function Damage in Chicken Kidney Exposed to Cr(VI)

Yue Wang #  1 , Xiaozhou Wang #  1 , Lumei Wang  1 , Guodong Cheng  2 , Meihua Zhang  1 , Yuxiao Xing  1 , Xiaona Zhao  1 , Yongxia Liu  2 , Jianzhu Liu  3
Affiliations

Mitophagy Induced by Mitochondrial Function Damage in Chicken Kidney Exposed to Cr(VI)

Yue Wang et al. Biol Trace Elem Res. 2021 Feb.

Abstract

Cr(VI) is a heavy metal environmental pollutant and carcinogen. Excessive Cr(VI) exposure injures kidneys. This study aimed to investigate mitophagy induced by mitochondrial function damage in chicken kidney exposed to Cr(VI). To explore the mechanism involved, we randomly divided 40 one-day-old Hy-line Brown cockerels into four groups, with each group exposed to different concentrations of Cr(VI), i.e., 0, 10, 30 and 50 mg kg-1, which were orally administered daily for 45 days. Excessive Cr(VI) increased tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and chemokine (C-X-C motif) ligand 1(CXCL1) expression and decreased Ca2+-adenosine triphosphatase (Ca2+-ATPase), Mg2+-ATPase and Na+/k+-ATPase activities in chicken kidney. Furthermore, Cr(VI) significantly increased reactive oxygen species (ROS) production and induced mitochondrial membrane potential (MMP) collapse and typical autophagosome formation. With the increase of Cr(VI) concentration, the Parkin translocation, value of LC3-II increased and decreased the content of p62/SQSTM1 and the translocase of outer mitochondrial membrane 20 (TOMM20). In summary, our findings explicated that mitochondrial function damage and mitophagy-related indicators were related to Cr(VI) concentration in chicken kidney.

Keywords: Chicken; Cr(VI); Kidney; Mitophagy; Parkin.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Kotyzova D, Hodková A, Bludovská M, Eybl V (2015) Effect of chromium (VI) exposure on antioxidant defense status and trace element homeostasis in acute experiment in rat. Toxicol Ind Health 31(11):1044–1050. https://doi.org/10.1177/0748233713487244 - DOI - PubMed
    1. De Flora S, D'Agostini F, Balansky R, Micale R, Baluce B, Izzotti A (2008) Lack of genotoxic effects in hematopoietic and gastrointestinal cells of mice receiving chromium(VI) with the drinking water. Mutat Res 659(1–2):60–67. https://doi.org/10.1016/j.mrrev.2007.11.005
    1. Thatoi H, Das S, Mishra J, Rath BP, Das N (2014) Bacterial chromate reductase, a potential enzyme for bioremediation of hexavalent chromium: a review. J Environ Manag 146:383–399. https://doi.org/10.1016/j.jenvman.2014.07.014 - DOI
    1. Roy RV, Pratheeshkumar P, Son YO, Wang L, Hitron JA, Divya SP, Zhang Z, Shi X (2016) Different roles of ROS and Nrf2 in Cr(VI)-induced inflammatory responses in normal and Cr(VI)-transformed cells. Toxicol Appl Pharmacol 307:81–90. https://doi.org/10.1016/j.taap.2016.07.016 - DOI - PubMed - PMC
    1. Lag M, Rodionov D, Ovrevik J, Bakke O, Schwarze PE, Refsnes M (2010) Cadmium-induced inflammatory responses in cells relevant for lung toxicity: expression and release of cytokines in fibroblasts, epithelial cells and macrophages. Toxicol Lett 193(3):252–260. https://doi.org/10.1016/j.toxlet.2010.01.015 - DOI - PubMed

LinkOut - more resources