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. 1988 Jun;5(6):369-71.
doi: 10.1023/a:1015959628008.

Carrier-mediated transport of baclofen across monolayers of bovine brain endothelial cells in primary culture

J B van Bree  1 , K L Audus, R T Borchardt
Affiliations

Carrier-mediated transport of baclofen across monolayers of bovine brain endothelial cells in primary culture

J B van Bree et al. Pharm Res. 1988 Jun.

Abstract

The mechanism of transport of baclofen, a centrally acting muscle relaxant, across the blood-brain barrier (BBB) was studied using an in vitro model. The model consisted of a monolayer of bovine brain endothelial cells grown in primary culture on a porous regenerated cellulose membrane. The transport of baclofen across the monolayer expressed time and concentration dependency and was saturable. Transport data were corrected for diffusion and fitted to the Michaelis-Menten Vmax model: Km = 58.5 microM, Vmax = 0.23 nmol/min. The results validate the use of the in vitro BBB model as described and support the hypothesis that baclofen penetrates the BBB by means of a carrier-mediated transport system.

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References

    1. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4544-8 - PubMed
    1. Fed Proc. 1986 Jun;45(7):2073-8 - PubMed
    1. Proc Soc Exp Biol Med. 1974 Dec;147(3):813-5 - PubMed
    1. J Neurochem. 1986 Aug;47(2):484-8 - PubMed
    1. J Cell Physiol. 1986 Aug;128(2):189-94 - PubMed

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