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. 2020 May 24;20(1):127.
doi: 10.1186/s12866-020-01793-9.

Profiles of Staphyloccocus aureus isolated from goat persistent mastitis before and after treatment with enrofloxacin

Magna Coroa Lima  1 , Mariana de Barros  1 , Thalita Moreira Scatamburlo  1 , Richard Costa Polveiro  1 , Laís Karolyne de Castro  1 , Samuel Henrique Sales Guimarães  1 , Sanely Lourenço da Costa  1 , Mateus Matiuzzi da Costa  2 , Maria Aparecida Scatamburlo Moreira  3
Affiliations

Profiles of Staphyloccocus aureus isolated from goat persistent mastitis before and after treatment with enrofloxacin

Magna Coroa Lima et al. BMC Microbiol. .

Abstract

Background: Staphylococcus aureus is one of the main causative agents of mastitis in small ruminants. Antimicrobial use is the major treatment, but there are many flaws linked to resistance, tolerance or persistence. This study aimed to verify changes in resistance, virulence and clonal profiles of S. aureus isolated from persistent mastitis goat milk before and after enrofloxacin treatment.

Results: MIC increased to at least one antimicrobial in S. aureus isolates after enrofloxacin treatment compared to before. The most detected resistance genes before and after treatment were tetK, tetM, and blaZ, with more resistance genes detected after enrofloxacin treatment (p < 0.05). Occasional variations in efflux system gene detection were observed before and after treatment. Nine virulence genes (hla, fnbA, fnbB, eta, etb, sea, sec, seh, and sej) were detected at both times, and between these, the hla and eta genes were detected more in isolates after treatment. All isolates of S. aureus belonged to the same sequence type (ST) 133, except for two S. aureus isolates prior to enrofloxacin treatment which were classified as ST5 and the other as a new one, ST4966. Isolates of S. aureus 4, 8, and 100 from before and after treatment had identical pulse types, while others obtained from other animals before and after treatment were classified into distinct pulse types.

Conclusion: There were occasional changes in the studied profiles of S. aureus isolated before and after treatment of animals with enrofloxacin, which may have contributed to the permanence of bacteria in the mammary gland, even when using traditional treatment, resulting in persistent mastitis.

Keywords: Caprine mastitis; Persistence; Small ruminants.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
Dendrogram of Pulsed Field Gel Electrophoresis of 18 Staphylococcus aureus isolates and your Sequence Types. Dendogram generated by the UPGMA/Dice (Bionumerics, Applied Maths). S. aureus isolates from the animals 4, 8 and 100, before and after treatment, presented identical pulse types (95% similarity and 5% tolerance and optimization). Sixteen isolates were within the same ST 133, the exceptions were S. aureus 5 and 101 from before treatment, which presented in ST 5 and a new ST 4966, respectively. c: isolates of goats with mastitis before treatment; p: isolates of goats with mastitis after treatment. Red dotted line: clusters (I-III); * Isolated not clustered in clusters
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