doi: 10.1002/art.1780200308.
An immunofluorescent method using Crithidia luciliae to detect antibodies to double-stranded DNA
- PMID: 324482
- DOI: 10.1002/art.1780200308
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An immunofluorescent method using Crithidia luciliae to detect antibodies to double-stranded DNA
Arthritis Rheum.
1977 Apr.
Abstract
The immunochemical specificity of the immunofluorescent Crithidia luciliae method for detection of antibodies to double-stranded DNA (dsDNA) was confirmed by demonstrating abolition of staining by DNase digestion and by absorption with dsDNA. This method was less sensitive than a Millipore filter method for detecting antibodies to DNA. It was positive only in subjects with systemic lupus erythematosus or drug-induced antinuclear factors. This technique appears suitable for study of the immunochemical characteristics fo antibodies to dsDNA.
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