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. 1977 Apr;20(3):811-4.
doi: 10.1002/art.1780200308.

An immunofluorescent method using Crithidia luciliae to detect antibodies to double-stranded DNA

W Crowe, I Kushner

An immunofluorescent method using Crithidia luciliae to detect antibodies to double-stranded DNA

W Crowe et al. Arthritis Rheum. 1977 Apr.

Abstract

The immunochemical specificity of the immunofluorescent Crithidia luciliae method for detection of antibodies to double-stranded DNA (dsDNA) was confirmed by demonstrating abolition of staining by DNase digestion and by absorption with dsDNA. This method was less sensitive than a Millipore filter method for detecting antibodies to DNA. It was positive only in subjects with systemic lupus erythematosus or drug-induced antinuclear factors. This technique appears suitable for study of the immunochemical characteristics fo antibodies to dsDNA.

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