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. 2020 Jul;17(7):681-684.
doi: 10.1038/s41592-020-0847-3. Epub 2020 May 25.

Illumination guidelines for ultrafast pump-probe experiments by serial femtosecond crystallography

Marie Luise Grünbein  1 , Miriam Stricker  1 , Gabriela Nass Kovacs  1 , Marco Kloos  1 , R Bruce Doak  1 , Robert L Shoeman  1 , Jochen Reinstein  1 , Sylvain Lecler  2 , Stefan Haacke  3 , Ilme Schlichting  4
Affiliations

Illumination guidelines for ultrafast pump-probe experiments by serial femtosecond crystallography

Marie Luise Grünbein et al. Nat Methods. 2020 Jul.

Abstract

Time-resolved crystallography with X-ray free-electron lasers enables structural characterization of light-induced reactions on ultrafast timescales. To be biologically and chemically relevant, such studies must be carried out in an appropriate photoexcitation regime to avoid multiphoton artifacts, a common issue in recent studies. We describe numerical and experimental approaches to determine how many photons are needed for single-photon excitation in microcrystals, taking into account losses by scattering.

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