Aureimonas psammosilene sp. nov., isolated from the roots of Psammosilene tunicoides

Abstract

One motile strain designated, YIM DR1026^T was isolated from the roots of Psammosilene tunicoides collected from Gejiu, Yunnan province, China. The cells of strain YIM DR1026^T were Gram-negative and short-rod shaped. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain YIM DR1026^T was a member of the genus Aureimonas and closely related to Aureimonas rubiginis (96.7%). DNA-DNA relatedness values between strain YIM 1026^T and Aureimonas rubiginis BCRC 80440^T was 38.2 ± 1.5%. The ANI value between YIM DR1026^T and other Aureimonas members were below the cut-off level (95-96%) recommended as the average nucleotide identity (ANI) criterion for interspecies identity. Strain YIM DR1026^T grew at 4-30 °C (optimum 28 °C), pH 4.0-9.0 (optimum pH 6.0-7.0) and tolerated NaCl (w/v) up to 1% (optimum 0%). Q-10 was sole the respiratory ubiquinone present in YIM DR1026^T. Polar lipids of strain YIM DR1026^T were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, sulfoquinovosyldiacylglycerol, unidentified aminolipid and unidentified polar lipid. The genomic G + C content was 64.6 mol%. The major fatty acids were C_18:1ω7c, C_16:0 and summed feature 3 (C_16:1ω7c/C_16:1ω6c). Based on phenotypic, phylogenetic, chemotaxonomic and genome comparison, strain YIM DR1026^T represents a novel species of the genus Aureimonas, for which the name Aureimonas psammosilene sp. nov. is proposed. The type strain is YIM DR1026^T (= KCTC 42691^T = NBRC 112412^T).

Keywords: Aureimonas psammosilene sp. nov.; Polyphasic taxonomy; Psammosilene tunicoides; Roots.