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Comment
. 2020 Jun;35(3):351-354.
doi: 10.1007/s12250-020-00236-z. Epub 2020 May 25.

A DNA Aptamer Based Method for Detection of SARS-CoV-2 Nucleocapsid Protein

Zhiqiang Chen  1   2 , Qihan Wu  3 , Jing Chen  3 , Xiaohua Ni  4 , Jianfeng Dai  5
Affiliations
Comment

A DNA Aptamer Based Method for Detection of SARS-CoV-2 Nucleocapsid Protein

Zhiqiang Chen et al. Virol Sin. 2020 Jun.
No abstract available

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Conflict of interest statement

The authors declare that they have no conflict of interest.

Figures

Fig. 1
Fig. 1
DNA aptamers specifically bind to SARS-CoV-2 N protein. A The expression and purification of SARS-CoV-2 N, S-RBD-Fc and 3CLpro proteins with a His-Ni–NTA system. B A sketch map for the ELAA. Briefly, the N protein was serially diluted and coated on the plates. After blocking, the N protein was detected with biotinylated ssDNA aptamers followed by HRP conjugated Avidin system. C Aptamer 1, 2 and 3 binds SARS-CoV-2 N protein in an Enzyme-Linked Aptamer Binding Assay (ELAA). BSA, SARS-CoV-2 S-RBD and 3CLpro proteins served as negative controls. Aptamer n.c. binds to none of the tested proteins. D Detection of N protein in diluted human serum by ssDNA aptamers. E ssDNA aptamer 2 specifically detects SARS-CoV-2 N protein in Western Blot assay. Antibody against N protein served as controls. Serially diluted N proteins (10 μg, 1μg, and 0.1μg) and 10 μg of 3CLpro protein were probed with N Aptamer 2 and N antibody, respectively. The data shown are representative of 3 independent experiments.
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