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. 2018 Dec;15(3):298-303.
doi: 10.4274/tjps.38258. Epub 2018 Nov 20.

Simultaneous Determination of Arbutin and Hydroquinone in Different Herbal Slimming Products Using Gas Chromatography-mass Spectrometry

Benan Dursunoğlu  1 , Hafize Yuca  1 , Zühal Güvenalp  1 , Sefa Gözcü  2 , Bilal Yilmaz  3
Affiliations

Simultaneous Determination of Arbutin and Hydroquinone in Different Herbal Slimming Products Using Gas Chromatography-mass Spectrometry

Benan Dursunoğlu et al. Turk J Pharm Sci. 2018 Dec.

Abstract

Objectives: The aim of our study was to develop a simple, precise, sensitive and specific method for the simultaneous determination of arbutin and hydroquinone in different herbal slimming products using GC-MS.

Materials and methods: The methanol and aqueous extracts of nine herbal slimming products in Turkey were evaluated for analysis of arbutin and hydroquinone using GC-MS method.

Results: The retention times of arbutin and hydroquinone were found as 11.32 and 5.44 min, respectively. The linear ranges in this method were 5-500 ng/mL for arbutin and hydroquinone, respectively. The intra- and inter-day precisions, expressed as the relative standard deviation, were less than 1.94 and 2.73%, determined from quality control samples for arbutin and hydroquinone, and accuracy was within 1.13 and 2.56% in terms of relative error, respectively. The limit of detection and quantification were 0.555 and 1.665 ng/mL for arbutin, and 0.031 and 0.093 ng/mL for hydroquinone, respectively.

Conclusion: The developed method can be used for routine quality control analysis of arbutin and hydroquinone in different herbal slimming products.

Keywords: Arbutin; Calluna vulgaris; GC-MS; herbal slimming products; hydroquinone.

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Conflict of interest statement

Conflict of Interest: No conflict of interest was declared by the authors.

Figures

Figure 1
Figure 1
Structural formula and derivatization mechanism of arbutin and hydroquinone
Figure 2
Figure 2
Mass spectrometry spectra of arbutin (A) and hydroquinone (B)
Figure 3
Figure 3
Gas chromatography-mass spectrometry chromatograms of arbutin (A) and hydroquinone (B) (500 ng/mL)
Figure 4
Figure 4
Typical chromatograms of methanol (A) and aqueous (B) extracts of product 9 (1.0 mg/mL)
See this image and copyright information in PMC

References

    1. Migas P, Baranowska MK. The significance of arbutin and its derivatives in therapy and cosmetics. Phytochem Lett. 2015;13:35–40.
    1. Thongchai W, Liawruangrath B, Liawruangrath S. Arbutin determination in medicinal plants and creams. Int J Cosmet Sci. 2009;31:87–96. - PubMed
    1. Pop C, Vlase L, Tamas M. Natural resources containing arbutin. Determination of arbutin in the leaves of Bergenia crassifolia (L.) Fritsch. acclimated in Romania. Not Bot Hort Agrobot Cluj. 2009;37:129–132.
    1. Jurica K, Karaconji IB, Segan S, Opsenica DM, Kremer D. Quantitative analysis of arbutin and hydroquinone in strawberry tree (Arbutus unedo L., Ericaceae) leaves by gas chromatography-mass spectrometry. Arh Hig Rada Toksikol. 2015;66:197–202. - PubMed
    1. Jeon JS, Kim BH, Lee SH, Kwon HJ, Bae HJ, Kim SK, Park JA, Shim JH, Abd El-Aty AM, Shin HC. Simultaneous determination of arbutin and its decomposed product hydroquinone in whitening creams using highperformance liquid chromatography with photodiode array detection: Effect of temperature and pH on decomposition. Int J Cosmet Sci. 2015;37:567–573. - PubMed

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