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. 2020 May 21;11(5):579.
doi: 10.3390/genes11050579.

Hepatic DNA Methylation in Response to Early Stimulation of Microbiota with Lactobacillus Synbiotics in Broiler Chickens

Aleksandra Dunislawska  1 , Anna Slawinska  1 , Maria Siwek  1
Affiliations

Hepatic DNA Methylation in Response to Early Stimulation of Microbiota with Lactobacillus Synbiotics in Broiler Chickens

Aleksandra Dunislawska et al. Genes (Basel). .

Abstract

DNA methylation inhibits DNA transcription by the addition of methyl residues to cysteine within the CpG islands of gene promoters. The process of DNA methylation can be modulated by environmental factors such as intestinal microbiota. In poultry, the composition of the intestinal microbiota can be stimulated by in ovo delivery of synbiotics. The present study aims to determine the effect of Lactobacillus synbiotics delivered in ovo on the level of hepatic DNA methylation in broiler chickens. In ovo stimulation was performed on day 12 of egg incubation. Bioactive compounds delivered in ovo included (S1)-Lactobacillus salivarius with GOS and (S2)-Lactobacillus plantarum with RFO. Samples were collected from six individuals from each group on day 42 post-hatching. DNA methylation of five genes selected on the basis of the transcriptome data were analyzed using the qMSP method. Significant changes were observed in DNA methylation of genes in liver including ANGPTL4 and NR4A3, after S2 delivery. The obtained results confirm that the downregulation of metabolic gene expression in the liver mediated by in ovo stimulation had epigenetic characteristics.

Keywords: gene silencing; gut–liver axis; prebiotic; probiotic.

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Conflict of interest statement

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyzes or interpretation of data; in the writing of the manuscript or in the decision to publish the results.

Figures

Figure 1
Figure 1
DNA methylation of the ANGPTL4, NR4A3, CYR61, KLHL6 and SYK genes in the liver. X-axis—groups: C—control, S1—Lactobacillus salivarius with GOS, S2—Lactobacillus plantarum with RFO; Y-axis—percentage of methylation. * p < 0.05 (n = 6).
See this image and copyright information in PMC

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