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. 2020 May 22;10(5):902.
doi: 10.3390/ani10050902.

Resilience Effects of SGK1 and TAP1 DNA Markers during PRRSV Outbreaks in Reproductive Sows

Marina Laplana  1 , Joan Estany  1 , Lorenzo José Fraile  1 , Ramona Natacha Pena  1
Affiliations

Resilience Effects of SGK1 and TAP1 DNA Markers during PRRSV Outbreaks in Reproductive Sows

Marina Laplana et al. Animals (Basel). .

Abstract

The porcine reproductive and respiratory syndrome virus (PRRSV) is a major infectious stressor that causes serious health problems and productivity drops. Based on previous genome-wide analyses, we selected SGK1 and TAP1 as candidate genes for resilience, and genotyped three mutations, including a 3'UTR variant SGK1_rs338508371 and two synonymous variants TAP1_rs1109026889 and TAP1_rs80928141 in 305 Landrace × Large White sows. All polymorphisms affected the reproductive performance in the outbreak, but not during the endemic phase, thereby indicating a potential use of these markers for resilience. Moreover, some genotypes were associated with a stable performance across PRRSV phases. Thus, in the outbreak, the SGK1_rs338508371 AA sows had less piglets born alive (p < 0.0001) and more stillborns (p < 0.05) while other sows were able to keep their productivity. During the outbreak, TAP1_rs80928141 GG sows had less piglets born alive (p < 0.05) and both TAP1 polymorphisms influenced the number of mummies in an additive manner (p < 0.05). Remarkably, TAP1_rs80928141 AA sows had around one mummy more than GG sows (p < 0.01). Resilience to PRRSV could be improved by including the SGK1 and TAP1 markers in crossbreeding and/or selection schemes, as they contribute to maintaining a stable number of piglets born alive and lost, particularly mummies, despite the outbreak.

Keywords: DNA markers; PRRSV; mummies; pigs; reproductive traits; resilience; sow.

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Conflict of interest statement

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

Figures

Figure 1
Figure 1
High-resolution melting (HRM) standardised genotyping images of the three tested polymorphisms, SGK1_rs338508371 (a), TAP1_rs1109026889 (b), and TAP1_rs80928141 (c) genotypes.
Figure 2
Figure 2
Least square means by PRRSV health status for the percentage of lost piglets per litter by SGK1_rs338508371 (a), TAP1_rs1109026889 (b), and TAP1_rs80928141 (c) genotypes. Error bars represent standard errors. Within markers, means with different letters differ significantly (p < 0.05).
See this image and copyright information in PMC

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