Inhibitory effects of selenium of the mutagenicity of 2-acetylaminofluorene (AAF) and AAF derivatives

Abstract

Selenium (Se) decreased the mutagenicity of 2-acetylaminofluorene (AAF), N-hydroxy-2-acetylaminofluorene (N-OH-AAF) and N-hydroxyaminofluorene (N-OH-AF) in the Salmonella typhimurium TA 1538 bacterial tester system. Metabolism of AAF and N-OH-AFF to the active mutagen, N-OH-AF, was accomplished by rat liver extracts. Graded decreases in mutagenicity with increasing Se concentrations were observed for each of the three mutagens. Se decreased the mutagenicity of AAF, N-OH-AAF and N-OH-AF to 65, 68 and 61% of their respective controls with mutagen alone. The effective molar ratios of Se to mutagen yielding these decreases were approximately 10:1 (Se:AAF), 10:1 (Se:N-OH-AAF) and 300:1 (Se:N-OH-AF). The largest Se effect observed was accomplished by a molar ratio of 100:1 (Se:N-OH-AAF) yielding 28% of the mutagenicity elicited by N-OH-AAF alone.