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. 2020 Oct;39(10):1997-2000.
doi: 10.1007/s10096-020-03930-8. Epub 2020 May 28.

The Allplex 2019-nCoV (Seegene) assay: which performances are for SARS-CoV-2 infection diagnosis?

Eric Farfour  1 , Philippe Lesprit  2 , Benoit Visseaux  3 , Tiffany Pascreau  2 , Emilie Jolly  2 , Nadira Houhou  3 , Laurence Mazaux  2 , Marianne Asso-Bonnet  2 , Marc Vasse  2 ; SARS-CoV-2 Foch Hospital study group
Affiliations

The Allplex 2019-nCoV (Seegene) assay: which performances are for SARS-CoV-2 infection diagnosis?

Eric Farfour et al. Eur J Clin Microbiol Infect Dis. 2020 Oct.

Abstract

Several commercial assays for SARS-CoV-2 RT-PCR are available but few of them were assessed. We evaluate the Allplex 2019-nCoV (Seegene) assay using 41 nasopharyngeal samples. The rates of agreement were 92.7% and 100% with the GeneFinder COVID-19 plus (Elitech) and the diagnosis of the infectious disease specialist respectively. Four samples display a Ct < 22.0 for the E and RdRp genes while the N gene was not detected, suggesting a variability of the viral sequence. There was no cross-reactivity with other respiratory viruses. The Allplex 2019-nCoV appears as a reliable method, but additional evaluations using more samples are needed. RT-PCR assays should probably include at least 2 viral targets.

Keywords: Allplex 2019-nCoV (Seegene); COVID-19; Nasopharyngeal swab; RT-PCR; SARS-CoV-2; Sensitivity.

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Conflict of interest statement

The authors declare that they have no conflict of interest.

References

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