Serum IgA, IgM, and IgG responses in COVID-19

doi:10.1038/s41423-020-0474-z

. 2020 Jul;17(7):773-775.

doi: 10.1038/s41423-020-0474-z. Epub 2020 May 28.

Serum IgA, IgM, and IgG responses in COVID-19

Huan Ma^#^{1

2}, Weihong Zeng^#^{1

2}, Hongliang He^#³, Dan Zhao², Dehua Jiang⁴, Peigen Zhou⁵, Linzhao Cheng^{6

7}, Yajuan Li⁸, Xiaoling Ma⁹, Tengchuan Jin¹⁰

Affiliations

¹ Department of Obstetrics and Gynecology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230001, Anhui, China.
² Hefei National Laboratory for Physical Sciences at Microscale, Laboratory of Structural Immunology, CAS Key Laboratory of Innate Immunity and Chronic Disease, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230027, Anhui, China.
³ Department of Infectious Diseases, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230001, Anhui, China.
⁴ Kangrun Biotech LTD, Guangzhou, 511400, Guangdong, China.
⁵ Department of Statistics, University of Wisconsin-Madison, Madison, WI, 53706, USA.
⁶ Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230027, Anhui, China.
⁷ Johns Hopkins University School of Medicine, Columbia, MD, USA.
⁸ Department of Clinical Laboratory, The First Affiliated Hospital of Anhui Medical University, Hefei, 230032, China. lyj106@ustc.edu.cn.
⁹ Department of Laboratory Medicine, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230001, Anhui, China. maxiaoling@ustc.edu.cn.
¹⁰ Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230027, Anhui, China. jint@ustc.edu.cn.

^# Contributed equally.

PMID: 32467617
PMCID: PMC7331804
DOI: 10.1038/s41423-020-0474-z

Serum IgA, IgM, and IgG responses in COVID-19

Huan Ma et al. Cell Mol Immunol. 2020 Jul.

. 2020 Jul;17(7):773-775.

doi: 10.1038/s41423-020-0474-z. Epub 2020 May 28.

Authors

Huan Ma^#^{1

2}, Weihong Zeng^#^{1

2}, Hongliang He^#³, Dan Zhao², Dehua Jiang⁴, Peigen Zhou⁵, Linzhao Cheng^{6

7}, Yajuan Li⁸, Xiaoling Ma⁹, Tengchuan Jin¹⁰

Affiliations

¹ Department of Obstetrics and Gynecology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230001, Anhui, China.
² Hefei National Laboratory for Physical Sciences at Microscale, Laboratory of Structural Immunology, CAS Key Laboratory of Innate Immunity and Chronic Disease, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230027, Anhui, China.
³ Department of Infectious Diseases, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230001, Anhui, China.
⁴ Kangrun Biotech LTD, Guangzhou, 511400, Guangdong, China.
⁵ Department of Statistics, University of Wisconsin-Madison, Madison, WI, 53706, USA.
⁶ Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230027, Anhui, China.
⁷ Johns Hopkins University School of Medicine, Columbia, MD, USA.
⁸ Department of Clinical Laboratory, The First Affiliated Hospital of Anhui Medical University, Hefei, 230032, China. lyj106@ustc.edu.cn.
⁹ Department of Laboratory Medicine, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230001, Anhui, China. maxiaoling@ustc.edu.cn.
¹⁰ Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230027, Anhui, China. jint@ustc.edu.cn.

^# Contributed equally.

PMID: 32467617
PMCID: PMC7331804
DOI: 10.1038/s41423-020-0474-z

No abstract available

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Conflict of interest statement

Dehua Jiang is an employee of Kangrun Biotech LTD (Guangzhou, China). Tengchuan Jin, Huan Ma, Weihong Zeng in USTC and Dehua Jiang have applied a joining patent related to the antibody detecting kits. Other authors declare that they have no conflicts of interest.

Figures

**Fig. 1**
Analysis of SARS-CoV-2 RBD-specific IgA, IgM, and IgG antibodies in 87 COVID-19 patients. Testing results of RBD-specific IgA (a), IgM (b), and IgG (c) kits using 330 healthy sera, 138 sera from other-type of patients who may interfere with the test, 15 sera of once-suspected pneumonia patients, and 216 sera of 87 qPCR-confirmed COVID-19 patients. RLU relative light units. Black bar indicates median values. The dotted line indicates the cut-off value for detecting of each isotypes of antibodies. d Sensitivity of RBD-specific IgA, IgM, and IgG detection in serum samples obtained at different periods after illness onset. The kinetics of anti-RBD IgA, IgM, and IgG levels in sera of COVID-19 patients at different time windows was analyzed (e). The median values of RLU were plotted for each isotype of three antibodies. Bars indicate median with interquartile ranges. f–h Serum antibody levels in healthy and three distinct severity groups of COVID-19 patients were analyzed. Healthy: 330 sera; Mild: 7 sera; Moderate: 44 sera; and Severe: 21 sera. The critically ill patients were included into the severe group. Only the data of serum antibody levels at 16–25 days after illness onset of COVID-19 patients were used

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References

1. Zhang W, et al. Molecular and serological investigation of 2019-nCoV infected patients: implication of multiple shedding routes. Emerg. Microbes Infect. 2020;9:386–389. doi: 10.1080/22221751.2020.1729071. - DOI - PMC - PubMed
1. To KK-W, et al. Temporal profiles of viral load in posterior oropharyngeal saliva samples and serum antibody responses during infection by SARS-CoV-2: an observational cohort study. Lancet Infect. Dis. 2020;20:565–574. doi: 10.1016/S1473-3099(20)30196-1. - DOI - PMC - PubMed
1. Zhao, J. et al. Antibody responses to SARS-CoV-2 in patients of novel coronavirus disease 2019. Clin. Infect. Dis. ciaa344. 10.1093/cid/ciaa344 (2020). - PMC - PubMed
1. Li, Z. et al. Development and clinical application of a rapid IgM‐IgG combined antibody test for SARS‐CoV‐2 infection diagnosis. J. Med. Virol. 10.1002/jmv.25727 (2020). - PMC - PubMed
1. Guo, L. et al. Profiling early humoral response to diagnose novel coronavirus disease (COVID-19). Clin. Infect. Dis. ciaa310. 10.1093/cid/ciaa310 (2020). - PMC - PubMed

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[1] Zhang W, et al. Molecular and serological investigation of 2019-nCoV infected patients: implication of multiple shedding routes. Emerg. Microbes Infect. 2020;9:386–389. doi: 10.1080/22221751.2020.1729071. - DOI - PMC - PubMed

[2] Zhang W, et al. Molecular and serological investigation of 2019-nCoV infected patients: implication of multiple shedding routes. Emerg. Microbes Infect. 2020;9:386–389. doi: 10.1080/22221751.2020.1729071. - DOI - PMC - PubMed

[3] To KK-W, et al. Temporal profiles of viral load in posterior oropharyngeal saliva samples and serum antibody responses during infection by SARS-CoV-2: an observational cohort study. Lancet Infect. Dis. 2020;20:565–574. doi: 10.1016/S1473-3099(20)30196-1. - DOI - PMC - PubMed

[4] To KK-W, et al. Temporal profiles of viral load in posterior oropharyngeal saliva samples and serum antibody responses during infection by SARS-CoV-2: an observational cohort study. Lancet Infect. Dis. 2020;20:565–574. doi: 10.1016/S1473-3099(20)30196-1. - DOI - PMC - PubMed

[5] Zhao, J. et al. Antibody responses to SARS-CoV-2 in patients of novel coronavirus disease 2019. Clin. Infect. Dis. ciaa344. 10.1093/cid/ciaa344 (2020). - PMC - PubMed

[6] Zhao, J. et al. Antibody responses to SARS-CoV-2 in patients of novel coronavirus disease 2019. Clin. Infect. Dis. ciaa344. 10.1093/cid/ciaa344 (2020). - PMC - PubMed

[7] Li, Z. et al. Development and clinical application of a rapid IgM‐IgG combined antibody test for SARS‐CoV‐2 infection diagnosis. J. Med. Virol. 10.1002/jmv.25727 (2020). - PMC - PubMed

[8] Li, Z. et al. Development and clinical application of a rapid IgM‐IgG combined antibody test for SARS‐CoV‐2 infection diagnosis. J. Med. Virol. 10.1002/jmv.25727 (2020). - PMC - PubMed

[9] Guo, L. et al. Profiling early humoral response to diagnose novel coronavirus disease (COVID-19). Clin. Infect. Dis. ciaa310. 10.1093/cid/ciaa310 (2020). - PMC - PubMed

[10] Guo, L. et al. Profiling early humoral response to diagnose novel coronavirus disease (COVID-19). Clin. Infect. Dis. ciaa310. 10.1093/cid/ciaa310 (2020). - PMC - PubMed

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Serum IgA, IgM, and IgG responses in COVID-19

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Serum IgA, IgM, and IgG responses in COVID-19

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