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. 2020 Sep;34(9):e23394.
doi: 10.1002/jcla.23394. Epub 2020 May 30.

Neuropilin1 silencing impairs the proliferation and migration of cells in pancreatic cancer

Li-Hong He  1   2 , Yong-Lin He  1   2 , Wen-Hang Zuo  3 , Yue Kang  1   2 , Huan Xue  1   2 , Ling-Yun Wang  1   2 , Yun-Liang Zhang  4 , Yong Meng  4
Affiliations

Neuropilin1 silencing impairs the proliferation and migration of cells in pancreatic cancer

Li-Hong He et al. J Clin Lab Anal. 2020 Sep.

Abstract

Background: Neuropilin1 (NRP1) participates in cancer cell proliferation, migration, and metastasis as a multifunctional co-receptor by interacting with multiple signal pathways, but few studies have addressed the precise function of NRP1 in pancreatic cancer (PACA) cells. We aimed to study whether NRP1 gene silencing involved in the proliferation and migration of PACA cells in vitro.

Methods: A lentiviral vector expressing NRP1 shRNA was constructed and transfected into human PACA cells (CFPAC-1 and PANC-1). The expression of NRP1 protein and mRNA was detected by Western blot and quantitative real-time polymerase chain reaction (qRT-PCR) assay, respectively. CCK-8 assay, wound healing assay, and transwell assay were conducted to examine the effect of NRP1 silencing on cells proliferation and migration capability.

Results: Results of qRT-PCR and Western blot showed successfully established, stably transfected shRNA-NRP1 cells in PACA cells. The proliferation capacity of PACA cells in NRP1 shRNA group was lower significantly than that in the negative control (NC) group (P < .05). The invasion and migration capability of PACA cells in NRP1 shRNA group was lower significantly than that in the NC group (P < .01).

Conclusions: NRP1-shRNA lentiviral interference vectors can effectively decrease NRP1 gene expression in PACA cells, thereby inhibiting cells proliferation and migration, which provides a basis for finding a valuable therapeutic target for PACA therapy.

Keywords: Neuropilin1; cell migration; cell proliferation; lentiviral interference; pancreatic cancer.

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Conflict of interest statement

The authors declare that there is no conflict of interest with respect to the research, authorship, and publication of this article.

Figures

Figure 1
Figure 1
Expression of green fluorescent protein in negative shRNA group and shNRP1 virus transfection group
Figure 2
Figure 2
Expression of NRP1 mRNA in (A) PANC‐1 and (B) CFPAC‐1 cells after transfection, as detected by qRT‐PCR
Figure 3
Figure 3
Expression of NRP1 protein in PANC‐1 and CFPAC‐1 cells after transfection, as detected with Western blot
Figure 4
Figure 4
Proliferation of (A) PANC‐1 and (B) CFPAC‐1 cells after NRP1 gene silencing, as detected by CCK8 assay
Figure 5
Figure 5
Migration of PANC‐1 and CFPAC‐1 cells after NRP1 gene silencing, as detected by transwell assay
Figure 6
Figure 6
Migration of (A) PANC‐1 and (B) CFPAC‐1 cells after NRP1 gene silencing, as detected by scratch test
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