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. 2020 Oct;228(2):596-608.
doi: 10.1111/nph.16713. Epub 2020 Jul 4.

ABI5 modulates seed germination via feedback regulation of the expression of the PYR/PYL/RCAR ABA receptor genes

Hongyun Zhao  1 , Kaili Nie  1 , Huapeng Zhou  2 , Xiaojing Yan  3 , Qidi Zhan  1 , Yuan Zheng  1 , Chun-Peng Song  1
Affiliations
Free article

ABI5 modulates seed germination via feedback regulation of the expression of the PYR/PYL/RCAR ABA receptor genes

Hongyun Zhao et al. New Phytol. 2020 Oct.
Free article

Abstract

As abscisic acid (ABA) receptors, PYR1/PYL/RCAR (PYLs) play important roles in ABA-mediated seed germination, but the regulation of PYLs in this process, especially at the transcriptional level, remains unclear. In this study, we found that expression of 11 of 14 PYLs changes significantly during seed germination and is affected by exogenous ABA. Two PYLs, PYL11 and PYL12, both of which are expressed specifically in mature seeds, positively modulate ABA-mediated seed germination. However, ABI5 was found to modulate the PYL11- and PYL12-mediated ABA response. In the abi5-7 mutant, ABA hypersensitivity caused by PYL11 and PYL12 overexpression was totally or partially blocked. By contrast, ABI5 regulates the expression of PYL11 and PYL12 by directly binding to their promoters. Moreover, the expression of eight other PYLs is also affected during the germination of abi5 mutants. Promoter analysis revealed that an ABI5-binding region is present next to the TATA box or initiator box. Together, our data demonstrate the role of PYL11 and PYL12 in seed germination. In addition, the identification of PYLs as targets of ABI5 reveals a role of ABI5 in the feedback regulation of ABA-mediated seed germination.

Keywords: ABI5; PYR1/PYL/RCARs; abscisic acid (ABA); feedback regulation; seed germination; transcriptional regulation.

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References

    1. Antoni R, Gonzalez-Guzman M, Rodriguez L, Rodrigues A, Pizzio GA, Rodriguez PL. 2012. Selective inhibition of clade A phosphatases type 2C by PYR/PYL/RCAR abscisic acid receptors. Plant Physiology 158: 970-980.
    1. Bueso E, Rodriguez L, Lorenzo-Orts L, Gonzalez-Guzman M, Sayas E, Muñoz-Bertomeu J, Ibañez C, Serrano R, Rodriguez PL. 2014. The single-subunit RING-type E3 ubiquitin ligase RSL1 targets PYL4 and PYR1 ABA receptors in plasma membrane to modulate abscisic acid signaling. The Plant Journal 80: 1057-1071.
    1. Carles C, Bies-Etheve N, Aspart L, Léon-Kloosterziel KM, Koornneef M, Echeverria M, Delseny M. 2002. Regulation of Arabidopsis thaliana Em genes: role of ABI5. The Plant Journal 30: 373-383.
    1. Chen HH, Li Q, Xu ZH, Zhu J-K. 2018. EL1-like casein kinases suppress ABA signaling and responses by phosphorylating and destabilizing the ABA receptors PYR/PYLs in Arabidopsis. Molecular Plant 11: 706-719.
    1. Clough SJ, Bent AF. 1998. Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. The Plant Journal 16: 735-743.

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