Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comment
. 2021 Jan;82(1):159-198.
doi: 10.1016/j.jinf.2020.05.061. Epub 2020 May 28.

Improved sensitivity using a dual target, E and RdRp assay for the diagnosis of SARS-CoV-2 infection: Experience at a large NHS Foundation Trust in the UK

Hayley Colton  1 , Michael Ankcorn  2 , Mehmet Yavuz  1 , Leeanne Tovey  1 , Alison Cope  1 , Mohammad Raza  1 , Alexander J Keeley  1 , Amy State  1 , Bozena Poller  1 , Matthew Parker  3 , Thushan I de Silva  4 , Cariad Evans  1
Affiliations
Comment

Improved sensitivity using a dual target, E and RdRp assay for the diagnosis of SARS-CoV-2 infection: Experience at a large NHS Foundation Trust in the UK

Hayley Colton et al. J Infect. 2021 Jan.
No abstract available

Keywords: COVID19; E gene; PCR; RdRp gene; SARS-CoV-2; diagnosis.

PubMed Disclaimer

Figures

Figure 1
Fig. 1
E and RdRp gene cycle threshold results in relation to symptom onset. E and RdRp amplification results plotted against days of symptom onset in 145 samples from 128 patients. Lowest CT values were seen around day 3 of symptoms, with mean RdRp CT higher at a given day compared to E gene CT value. The lines represent the smoothed conditional mean with 95% confidence intervals in the grey bars. E, envelope gene; RdRp, RNA-dependent RNA-polymerase.
See this image and copyright information in PMC

Comment on

References

    1. Hao W, Li M. Clinical features of atypical 2019 novel coronavirus pneumonia with an initially negative RT-PCR assay. J Infect. 2020;80(6):671–693. - PMC - PubMed
    1. Udugama B, Kadhiresan P, Kozlowski HN. Diagnosing COVID-19: The Disease and Tools for Detection. ACS Nano. 2020 - PubMed
    1. Questions and answers regarding laboratory topics on SARS-CoV-2. Available at:https://www.ecdc.europa.eu/en/all-topics-z/coronavirus/threats-and-outbr.... Accessed 19th April.
    1. Corman VM, Landt O, Kaiser M. Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Euro Surveill. 2020;25(3) - PMC - PubMed
    1. Sawicki SG, Sawicki DL, Siddell SG. A contemporary view of coronavirus transcription. J Virol. 2007;81(1):20–29. - PMC - PubMed

Substances