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. 2020 Aug;251(4):440-451.
doi: 10.1002/path.5478. Epub 2020 Jun 30.

Histological 3D reconstruction and in vivo lineage tracing of the human endometrium

Nicola Tempest  1   2 , Marnix Jansen  3 , Ann-Marie Baker  4 , Christopher J Hill  2 , Mike Hale  5 , Derek Magee  6   7 , Darren Treanor  5   8   9 , Nicholas A Wright  4 , Dharani K Hapangama  1   2
Affiliations
Free article

Histological 3D reconstruction and in vivo lineage tracing of the human endometrium

Nicola Tempest et al. J Pathol. 2020 Aug.
Free article

Abstract

Regular menstrual shedding and repair of the endometrial functionalis is unique to humans and higher-order primates. The current consensus postulates endometrial glands to have a single-tubular architecture, where multi-potential stem cells reside in the blind-ending glandular-bases. Utilising fixed samples from patients, we have studied the three-dimensional (3D) micro-architecture of the human endometrium. We demonstrate that some non-branching, single, vertical functionalis glands originate from a complex horizontally interconnecting network of basalis glands. The existence of a multipotent endometrial epithelial stem cell capable of regenerating the entire complement of glandular lineages was demonstrated by in vivo lineage tracing, using naturally occurring somatic mitochondrial DNA mutations as clonal markers. Vertical tracking of mutated clones showed that at least one stem-cell population resides in the basalis glands. These novel findings provide insight into the efficient and scar-less regenerative potential of the human endometrium. © 2020 The Authors. The Journal of Pathology published by John Wiley & Sons Ltd on behalf of Pathological Society of Great Britain and Ireland.

Keywords: 3D reconstruction; endometrium; lineage tracing; regeneration; stem cells.

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References

    1. Valentijn AJ, Palial K, Al-Lamee H, et al. SSEA-1 isolates human endometrial basal glandular epithelial cells: phenotypic and functional characterization and implications in the pathogenesis of endometriosis. Hum Reprod 2013; 28: 2695-2708.
    1. Nguyen HPT, Xiao L, Deane JA, et al. N-cadherin identifies human endometrial epithelial progenitor cells by in vitro stem cell assays. Hum Reprod 2017; 32: 2254-2268.
    1. Kim JY, Tavare S, Shibata D. Counting human somatic cell replications: methylation mirrors endometrial stem cell divisions. Proc Natl Acad Sci U S A 2005; 102: 17739-17744.
    1. Tempest N, Maclean A, Hapangama DK. Endometrial stem cell markers: current concepts and unresolved questions. Int J Mol Sci 2018; 19.
    1. Tanaka M, Kyo S, Kanaya T, et al. Evidence of the monoclonal composition of human endometrial epithelial glands and mosaic pattern of clonal distribution in luminal epithelium. Am J Pathol 2003; 163: 295-301.

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