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. 2018;35(1):5-15.
doi: 10.36141/svdld.v35i1.5834. Epub 2018 Apr 28.

Identification of apolipoprotein A-I in BALF as a biomarker of sarcoidosis

Yoshihisa Nukui  1 , Yasunari Miyazaki  1 , Kozo Suhara  1 , Tsukasa Okamoto  1 , Haruhiko Furusawa  1 , Naohiko Inase  1
Affiliations

Identification of apolipoprotein A-I in BALF as a biomarker of sarcoidosis

Yoshihisa Nukui et al. Sarcoidosis Vasc Diffuse Lung Dis. 2018.

Abstract

Background: Sarcoidosis goes into remission in two-thirds of patients with sarcoidosis, but about 20 % of patients develop pulmonary fibrosis. The mechanisms of pulmonary fibrosis in sarcoidosis and differences in pathogenesis between clinical stages are still unclear. Objectives: The aim of this study was investigating proteins associated with clinical stages by comparing bronchoalveolar lavage fluid (BALF) protein between stage I and stage IV using proteome analysis. Methods: Proteomic differences in BALF were compared between stage I and stage IV by examining BALF from 8 stage I patients and 5 stage IV patients by two-dimensional gel electrophoresis and mass spectrometry. Results: In individual comparisons of BALF samples, the levels of apolipoprotein (Apo) A-I fragment, fibrinogen γ chain, calcyphosine, complement C3, and surfactant protein A were significantly higher in stage I than in stage IV. In contrast, none of the proteins examined significantly higher in stage IV than in stage I. To confirm the results of Apo A-I in the BALF proteome, we performed enzyme-linked immunosorbent assay (ELISA) in a larger group. The concentration of BALF Apo A-I was significantly higher in stage I patients than in stage IV patients (0.70 [0.13-0.89] vs. 0.15 [0.08-0.21] ng/μg protein, p=0.003). Conclusion: The involvement of BALF Apo A-I in sarcoidosis may differ between stage I and stage IV. (Sarcoidosis Vasc Diffuse Lung Dis 2018; 35: 5-15).

Keywords: apolipoprotein A-I; bronchoalveolar lavage fluid; interstitial pneumonia; proteomics; sarcoidosis.

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Figures

Fig. 1.
Fig. 1.
Two-Dimensional Electrophoresis (2-DE) of BALF from sarcoidosis patient with stage I (A) stained by SYPRO-Ruby. Numbers indicate proteins, as listed in Table 2. Representative 2-DE patterns of BALF from stage I (B) and stage IV (C). The square area is magnified in Fig. 1D. Apo A-I fragments (spot“ a ” - “ c ”) and Apo A-I (spot“ d ” - “ e ”) were shown as five spots
Fig. 2.
Fig. 2.
The normalized BALF Apo A-I concentration with total protein in sarcoidosis with stage I (Sar. I), sarcoidosis with stage IV (Sar. IV), rheumatoid arthritis-associated interstitial lung disease with organizing pneumonia pattern (RA-ILD OP), rheumatoid arthritis-associated interstitial lung disease with usual interstitial pneumonia pattern (RA-ILD UIP), idiopathic pulmonary fibrosis (IPF), and healthy volunteers (HV). * p<0.05
Fig. 3.
Fig. 3.
Correlation between normalized concentration of BALF Apo A-I and the percentage of lymphocytes in BALF in stage I sarcoidosis
See this image and copyright information in PMC

References

    1. Statement on sarcoidosis. Joint Statement of the American Thoracic Society (ATS), the European Respiratory Society (ERS) and the World Association of Sarcoidosis and Other Granulomatous Disorders (WASOG) adopted by the ATS Board of Directors and by the ERS Executive Committee, February 1999. AJRCCM. 1999;160:736–55. - PubMed
    1. Van Lenten BJ, Navab M, Anantharamaiah GM, Buga GM, Reddy ST, Fogelman AM. Multiple indications for anti-inflammatory apolipoprotein mimetic peptides. Curr Opin Investig Drugs. 2008;9:1157–62. - PMC - PubMed
    1. Li X, Chyu KY, Faria Neto JR, et al. Differential effects of apolipoprotein A-I-mimetic peptide on evolving and established atherosclerosis in apolipoprotein E-null mice. Circulation. 2004;110:1701–5. - PubMed
    1. Kim TH, Lee YH, Kim KH, et al. Role of lung apolipoprotein A-I in idiopathic pulmonary fibrosis: antiinflammatory and antifibrotic effect on experimental lung injury and fibrosis. AJRCCM. 2010;182:633–42. - PubMed
    1. Park SW, Lee EH, Lee EJ, et al. Apolipoprotein A1 potentiates lipoxin A4 synthesis and recovery of allergen-induced disrupted tight junctions in the airway epithelium. Clin Exp Allergy. 2013;43:914–27. - PubMed

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