Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020;8(6):239-246.
doi: 10.1080/21663831.2020.1744758. Epub 2020 Apr 7.

Effect of surface grain boundary density on preosteoblast proliferation on titanium

Terry C Lowe  1 , Rebecca A Reiss  2 , Patrick E Illescas  2 , Casey F Davis  1 , Melanie C Connick  2 , Johnny A Sena  3
Affiliations

Effect of surface grain boundary density on preosteoblast proliferation on titanium

Terry C Lowe et al. Mater Res Lett. 2020.

Abstract

Studies since 2004 have shown that the cytocompatibility of ultrafine grain (UG) commercial purity (CP) titanium exceeds that of coarse grain (CG) CP titanium (Ti) by 30% to 20-fold. To isolate the factors affecting this large reported variability of CP titanium's cytocompatibility, discs of UG and CG titanium were fabricated with controlled texture and roughness. The discs were seeded with MC3T3-E1 pre-osteoblastic cells and cultured for 72 h. The proliferation of cells on polished UG-Ti exceeded unpolished CG-Ti 3.04-fold. Cell proliferation was found to correlate with a new biophysical parameter, the average grain boundary length per surface-attached cell.

Keywords: Cell proliferation; grain boundary density; ultrafine grain titanium.

PubMed Disclaimer

Conflict of interest statement

Disclosure statement No potential conflict of interest was reported by the author(s).

Figures

Figure 1.
Figure 1.
Rod/extrusion axis inverse pole figure maps of (a) CG-Ti (1000x magnification) and (b) UG-Ti (20,000x magnification); and corresponding rod/extrusion axis inverse pole figures for (c) CG-Ti and (d) UG-Ti.
Figure 2.
Figure 2.
Grain area distributions for CG-Ti and UG-Ti.
Figure 3.
Figure 3.
Fluorescent images of mouse preosteoblasts after 72 h for (a) CG-Ti unpolished, (b) CG-Ti polished, (c) UG-Ti unpolished, and(d) UG-Ti polished. Mean cell counts (e) for all four states, with p-values for significant pairwise comparisons shown.
Figure 4.
Figure 4.
Relationship between the average number of attached MC3T3-E1 cells and average arithmetic mean roughness Ra.
Figure 5.
Figure 5.
Attached number of MC3T3-E1 cells after 72 h increases with GBL/Cell, the average length of grain boundaries per attached cell on the substrate surface.
See this image and copyright information in PMC

References

    1. Buser D, Sennerby L, De Bruyn H. Modern implant dentistry based on osseointegration: 50 years of progress, current trends and open questions. Periodontol 2000. 2017;73(1):7–21. - PubMed
    1. Rack H, Qazi J. Titanium alloys for biomedical applications. Mater Sci Eng C. 2006;26:1269–1277.
    1. Kunčická L, Kocich R, Lowe TC. Advances in metals and alloys for joint replacement. Prog Mater Sci. 2017;88:232–280.
    1. Wang M Surface modification of metallic biomaterials for orthopaedic applications. Mater Sci. 2009;618–619:285–290.
    1. Valiev RZ, Estrin Y, Horita Z, et al. Fundamentals of Superior Properties in Bulk NanoSPD Materials. Mater Res Lett. 2016;4(1):1–21.

LinkOut - more resources