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. 2020 May 29;43(2):e20180380.
doi: 10.1590/1678-4685-GMB-2018-0380. eCollection 2020.

Genetic differences among Moraxella bovis and Moraxella bovoculi isolates from infectious bovine keratoconjunctivitis (IBK) outbreaks in southern Brazil

Helena Brocardo Comin  1 , Robert Domingues  2 , Emanuelle Baldo Gaspar  2 , João Rodrigo Gil De Los Santos  3 , Fernando Flores Cardoso  2   3
Affiliations

Genetic differences among Moraxella bovis and Moraxella bovoculi isolates from infectious bovine keratoconjunctivitis (IBK) outbreaks in southern Brazil

Helena Brocardo Comin et al. Genet Mol Biol. .

Abstract

The objective of this study was to evaluate the genetic diversity of Moraxella bovis and Moraxella bovoculi bacteria isolated from infectious bovine keratoconjunctivitis (IBK) outbreaks in the state of Rio Grande do Sul, Brazil. The genetic diversity among Moraxella spp. was evaluated by RAPD-PCR, JWP1-JWOPA07-PCR, ERIC-PCR and by sequencing the 16S-23S intergenic regions. Based on the dendrogram, two genetically differentiated clades were observed; 14 isolates were classified as M. bovis and 17 as M. bovoculi. Genetic distances between the M. bovis samples ranged from 0.0379 to 0.4285, while for M. bovoculi the dissimilarities ranged from zero to 0.7297. Alternatively, based on sequencing analyses of the 16S-23S intergenic region, M. bovis and M. bovoculi isolates were grouped into the same two different clades, but it was not possible to differentiate between isolates within clades. PCR techniques were demonstrated to be a satisfactory tool to unravel the genetic variability among Moraxella spp., while sequencing of the 16S-23S intergenic region was only able to differentiate two species of the Moraxella genus. Despite sampling geographically close regions, we demonstrate considerable genetic diversity in M. bovis and M. bovoculi strains and genetically distinct M. bovis strains co-infecting the same animal.

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Conflict of interest statement

Conflict of Interest: The authors declare that there is no conflict of interest associated with this publication.

Figures

Figure 1
Figure 1. Example of RAPD technique using a single primer (OPA-03). After RAPD-PCR, ten samples of M. bovis (left side) and nine samples of M. bovoculi (right side) were run in an agarose gel and stained with ethidium bromide.
Figure 2
Figure 2. Dendrogram representing the genetic relationships between M. bovis and M. bovoculi isolates by the UPGMA method. Bootstrap values represented in the tree (2000 bootstrap repetitions).
Figure 3
Figure 3. Phylogenetic tree of the ITS region of the M. bovis and M. bovoculi isolates inferred by the maximum likelihood algorithm.
See this image and copyright information in PMC

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