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. 2021 Jan;93(1):48-50.
doi: 10.1002/jmv.26112. Epub 2020 Jun 9.

Amplification of human β-glucuronidase gene for appraising the accuracy of negative SARS-CoV-2 RT-PCR results in upper respiratory tract specimens

Eliseo Albert  1 , Blanca Ferrer  2 , Ignacio Torres  1 , Alicia Serrano  2 , María J Alcaraz  1 , Javier Buesa  1   3 , Carlos Solano  2   4 , Javier Colomina  1 , Felipe Bueno  1 , Dixie Huntley  1 , Beatriz Olea  1 , Arantxa Valdivia  1 , David Navarro  1   3
Affiliations

Amplification of human β-glucuronidase gene for appraising the accuracy of negative SARS-CoV-2 RT-PCR results in upper respiratory tract specimens

Eliseo Albert et al. J Med Virol. 2021 Jan.
No abstract available

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Conflict of interest statement

The authors declare no conflicts of interests.

Figures

Figure 1
Figure 1
SARS‐CoV‐2 RT‐PCR and serology testing results in patients with clinical suspicion of Covid‐19 admitted to Hospital Clínico Universitario of Valencia during the study period. Nasopharyngeal or oropharyngeal swabs were obtained with flocked swabs in universal transport medium (Beckton Dickinson, Sparks, MD or Copan Diagnostics, Murrieta, CA) and conserved at 4°C until processed (within 6 hours). Nucleic acid extraction was performed using the Qiagen EZ1 Viral extraction kit or the DSP virus Pathogen Minikit on the EZ1 or QiaSymphony Robot instruments (Qiagen, Valencia, CA), respectively. Commercially available PCR assays used for SARS‐CoV‐19 testing included the LightMix Modular SARS‐CoV‐2 (COVID‐19) E‐gene/LightMix Modular SARS‐CoV‐2 (COVID‐19) RdRP gene from TIB MOLBIOL GmHD, distributed by Roche Diagnostics (Pleasanton, CA) on the Light Cycler 2.0 instrument, the SARS‐CoV‐2 Real‐time PCR Kit from Vircell Diagnostics (Granada, Spain), or the Realquality RQ‐2019‐nCoV from AB Analitica (Padua, Italy), both on the Applied Biosystems 7500 instrument and the SARS‐CoV‐2 (S gene)–BD Max System (Viasure Real‐Time PCR Detection Kits; CerTest, Zaragoza, Spain). Results were interpreted according to the respective manufacturer's instructions. Sera from these patients were drawn at a median of 12 days (range, 10‐21 days) after admission. The presence of either SARS‐CoV‐2 IgM (determined by the MAGLUMI 2019‐nCoV SARS‐CoV‐2‐ IgM assay on the fully automated Maglumi analyzers‐Snibe—Shenzhen New Industries Biomedical Engineering Co, Ltd, Shenzhen, China), IgG (Euroimmun anti‐SARS‐CoV‐2 IgG assay; Euroimmun, Luebeck, Germany), or both confirmed diagnosis of Covid‐19. Covid‐19, coronavirus disease; IgG, immunoglobulin G; IgM, immunoglobulin M; RT‐PCR, real‐time reverse transcription polymerase chain reaction; SARS‐CoV‐2, severe acute respiratory syndrome coronavirus 2
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