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Review
. 2020 Jun 16;59(23):2122-2124.
doi: 10.1021/acs.biochem.0c00411. Epub 2020 Jun 3.

Ramping Recombinant Protein Expression in Bacteria

Walter J Zahurancik, Blake E Szkoda, Lien B Lai, Venkat Gopalan
Review

Ramping Recombinant Protein Expression in Bacteria

Walter J Zahurancik et al. Biochemistry. .
No abstract available

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Conflict of interest statement

The authors declare no competing financial interest.

Figures

Figure 1.
Figure 1.
Codons 3–5 and their amino acids act as a translational ramp in a sequence-dependent manner. Low-efficiency sequences are associated with ribosomal pausing at codon 4 or 5 and termination of protein synthesis. In contrast, AU-rich sequences and the specified dipeptides (inset) favor high efficiency, leading to processive translation through codons 3–5 and an increased level of full-length polypeptide synthesis.
See this image and copyright information in PMC

References

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