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. 1977 Jun;130(3):1393-6.
doi: 10.1128/jb.130.3.1393-1396.1977.

Deoxyribonucleic acid strand breaks during freeze-drying and their repair in Escherichia coli

T Ohnishi, Y Tanaka, M Yoh, Y Takeda, T Miwatani

Deoxyribonucleic acid strand breaks during freeze-drying and their repair in Escherichia coli

T Ohnishi et al. J Bacteriol. 1977 Jun.

Abstract

Freeze-drying of Escherichia coli cells caused strand breaks of deoxyribonucleic acid (DNA) in both radiation-sensitive and -resistant strains. However, in the radiation-resistant strain E. coli B/r the damaged DNA was repaired after rehydration, whereas in the radiation-sensitive strain E. coli Bs-1 the damaged DNA was not repaired and the DNA was degraded. Repeated freeze-drying did not break the damaged DNA into smaller pieces.

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