Visual detection of high-risk HPV16 and HPV18 based on loop-mediated isothermal amplification

Zhaoxuan Fan¹, Xiao Feng², Weifei Zhang³, Nan Li³, Xueji Zhang⁴, Jin-Ming Lin⁵

Affiliations

¹ Research Center for Bioengineering and Sensing Technology, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, PR China; Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, MOE Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing, 100084, PR China.
² Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, MOE Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing, 100084, PR China; State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, PR China.
³ Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, MOE Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing, 100084, PR China.
⁴ Research Center for Bioengineering and Sensing Technology, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, PR China; School of Biomedical Engineering, Shenzhen University, Shenzhen, Guangdong 518060, China. Electronic address: zhangxueji@szu.edu.cn.
⁵ Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, MOE Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing, 100084, PR China. Electronic address: jmlin@mail.tsinghua.edu.cn.

PMID: 32498911
DOI: 10.1016/j.talanta.2020.121015

Visual detection of high-risk HPV16 and HPV18 based on loop-mediated isothermal amplification

Zhaoxuan Fan et al. Talanta. 2020.

. 2020 Sep 1:217:121015.

doi: 10.1016/j.talanta.2020.121015. Epub 2020 Apr 16.

Authors

Zhaoxuan Fan¹, Xiao Feng², Weifei Zhang³, Nan Li³, Xueji Zhang⁴, Jin-Ming Lin⁵

Affiliations

¹ Research Center for Bioengineering and Sensing Technology, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, PR China; Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, MOE Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing, 100084, PR China.
² Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, MOE Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing, 100084, PR China; State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, PR China.
³ Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, MOE Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing, 100084, PR China.
⁴ Research Center for Bioengineering and Sensing Technology, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, PR China; School of Biomedical Engineering, Shenzhen University, Shenzhen, Guangdong 518060, China. Electronic address: zhangxueji@szu.edu.cn.
⁵ Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, MOE Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing, 100084, PR China. Electronic address: jmlin@mail.tsinghua.edu.cn.

PMID: 32498911
DOI: 10.1016/j.talanta.2020.121015

Abstract

Increased adoption of HPV testing is expected in light of a growing global awareness of women's health and the recent launch of cervical cancer vaccines. Testing approaches must be both easy to implement, and offer intelligible representation of amplification results. Here, we introduce a simple, rapid, and visual HPV detection method based on a loop-mediated isothermal amplification (LAMP) assay. The visual LAMP assay can be successfully applied to amplify and differentiate the high-risk samples of HPV16 and HPV18, and results can be discriminated via the naked eye, without costly specialized apparatus. This HPV testing method has been evaluated with clinical samples and exhibited excellent reliability, as verified by polymerase chain reaction-microchip electrophoresis (PCR-MCE), and has a reduced false negative rate compared with cytological methods. The LAMP-based platforms with their facile operation and visual results possess great potential for on-site cervical cancer monitoring.

Keywords: Cervical cancer; Human papilloma virus; Isothermal amplification; Microchip electrophoresis; Visual LAMP detection.

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Visual detection of high-risk HPV16 and HPV18 based on loop-mediated isothermal amplification

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Visual detection of high-risk HPV16 and HPV18 based on loop-mediated isothermal amplification

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