PAM-less is more

Lei Tang¹

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Comment

Lei Tang. Nat Methods. 2020 Jun.

. 2020 Jun;17(6):559.

doi: 10.1038/s41592-020-0861-5.

Lei Tang¹

No abstract available

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Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants.
Walton RT, Christie KA, Whittaker MN, Kleinstiver BP. Walton RT, et al. Science. 2020 Apr 17;368(6488):290-296. doi: 10.1126/science.aba8853. Epub 2020 Mar 26. Science. 2020. PMID: 32217751 Free PMC article.
An engineered ScCas9 with broad PAM range and high specificity and activity.
Chatterjee P, Jakimo N, Lee J, Amrani N, Rodríguez T, Koseki SRT, Tysinger E, Qing R, Hao S, Sontheimer EJ, Jacobson J. Chatterjee P, et al. Nat Biotechnol. 2020 Oct;38(10):1154-1158. doi: 10.1038/s41587-020-0517-0. Epub 2020 May 11. Nat Biotechnol. 2020. PMID: 32393822

1. Walton, R. T. et al. Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants. Science 368, 290–296 (2020). - DOI
1. Chatterjee, P. et al. An engineered ScCas9 with broad PAM range and high specificity and activity. Nat. Biotechnol. https://doi.org/10.1038/s41587-020-0517-0 (2020).