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. 2020 May 29;2020(3):hoaa020.
doi: 10.1093/hropen/hoaa020. eCollection 2020.

ESHRE PGT Consortium and SIG Embryology good practice recommendations for polar body and embryo biopsy for PGT

ESHRE PGT Consortium and SIG-Embryology Biopsy Working Group; Georgia Kokkali  1 , Giovanni Coticchio  2 , Fernando Bronet  3 , Catherine Celebi  4 , Danilo Cimadomo  5 , Veerle Goossens  6 , Joanna Liss  7   8 , Sofia Nunes  9 , Ioannis Sfontouris  10   11 , Nathalie Vermeulen  6 , Elena Zakharova  12 , Martine De Rycke  13   14
Affiliations

ESHRE PGT Consortium and SIG Embryology good practice recommendations for polar body and embryo biopsy for PGT

ESHRE PGT Consortium and SIG-Embryology Biopsy Working Group et al. Hum Reprod Open. .

Abstract

The field of preimplantation genetic testing (PGT) is evolving fast, and best practice advice is essential for regulation and standardisation of diagnostic testing. The previous ESHRE guidelines on best practice for PGD, published in 2005 and 2011, are considered outdated, and the development of new papers outlining recommendations for good practice in PGT was necessary.The current paper provides recommendations on the technical aspects of embryo biopsy and covers recommendations on the biopsy procedure, cryopreservation and laboratory issues and training, in addition to technical aspects and strengths and limitations specific for currently used techniques at different stages (polar body, cleavage stage and blastocyst biopsy). Furthermore, alternative sampling methods are briefly described.This paper is one of a series of four papers on good practice recommendations on PGT. The other papers cover the organisation of PGT, and the different technical aspects of PGT for monogenic/single-gene defects (PGT-M) and PGT for chromosomal structural rearrangements/aneuploidies (PGT-SR/PGT-A).Together, these papers should assist everyone interested in PGT in developing the best laboratory and clinical practice possible.

Keywords: ESHRE; biopsy; good practice; preimplantation genetic testing; tubing.

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Figures

Figure 1
Figure 1
Overview of the IVF/PGT process, and how all aspects are covered by one of the four recommendations papers. IVF: in vitro fertilisation, PGT: preimplantation genetic testing.
Figure 2
Figure 2
Methods of oocyte and embryo biopsy.
Figure 3
Figure 3
Methods of blastocyst biopsy. ZP: zona pellucida, TE: trophectoderm.
Figure 4
Figure 4
Alternative biopsy and sampling methods.
See this image and copyright information in PMC

References

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    1. ESHRE Guideline Group on Good Practice in IVF Labs, De los Santos MJ, Apter S, Coticchio G, Debrock S, Lundin K, Plancha CE, Prados F, Rienzi L, Verheyen G. et al. Revised guidelines for good practice in IVF laboratories (2015). Hum Reprod 2016;31:685–686. - PubMed
    1. ESHRE PGT Consortium Steering committee, Carvalho F, Coonen E, Goossens V, Kokkali G, Rubio C, Meijer-Hoogeveen M, Moutou C, Vermeulen N, De Rycke M. ESHRE PGT Consortium good practice recommendations for the organisation of preimplantation genetic testing. Hum Reprod Open 2020. doi: 10.1093/hropen/hoaa021. - PMC - PubMed
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