The prognostic and diagnostic values of MicroRNA-10b in gastric cancer: A comprehensive study based on meta-analysis and TCGA database

Abstract

We conducted a study to evaluate the prognostic and diagnostic values of microRNA-10b (miR-10b) in gastric cancer (GC) based on meta-analysis and TCGA database. Relevant studies were searched in English and Chinese database and meta-analysis was conducted on Stata 12.0. The expression value of miR-10b and clinical parameters of GC patients were downloaded from TCGA database, and relevant analyses were conducted on SPSS. High expression of miR-10b was linked with unfavorable overall survival (OS) in GC (HR = 1.572, 95% CI: 1.240-1.992, P < .001). However, the meta-analysis was significant for patients in early stage, but not for patients in advanced stage. The expression of miR-10b-3p was significantly lower in cancer tissue compared with adjacent tissue (P < .001). Meanwhile, the area under the ROC curve (AUC) value was 0.652 (0.562-0.742), P = .001. Disease-free survival analysis showed increasing miR-10b-5p was correlated with worse survival outcome (HR = 2.366, 95% CI: 1.414-3.959, P = .001). In conclusion, miR-10b acts as a tumor suppressor with prognostic and diagnostic values for GC.

Figure 1

Flow chart showing the selection process for the including studies.

Figure 2

(A) Forest plot for the association between miR-10b expression and OS of GC; (B) Forest plot for the association between miR-10b expression and DFS of GC; (C) Forest plot for the association between miR-10b expression and OS of GC in qRT-PCR group; (D) Forest plot for the association between miR-10b expression and OS of GC in ISH group.

Figure 3

Forest plot for the association between miR-10b expression and OS of GC in different stages. (A) stage I; (B) stage II; (C) stage III; (D) stage IV.

Figure 4

(A) DFS of miR-10b-5p for GC; (B) OS of miR-10b-5p for GC; (C) OS of miR-10b-3p for GC; (D) DFS of miR-10b-3p for GC.