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. 2020 Sep;27(25):32017-32033.
doi: 10.1007/s11356-020-08916-9. Epub 2020 Jun 6.

Antiproliferative and apoptogenic effects of Cassia fistula L. n-hexane fraction against human cervical cancer (HeLa) cells

Sandeep Kaur  1   2 , Kritika Pandit  1 , Madhu Chandel  2 , Satwinderjeet Kaur  3
Affiliations

Antiproliferative and apoptogenic effects of Cassia fistula L. n-hexane fraction against human cervical cancer (HeLa) cells

Sandeep Kaur et al. Environ Sci Pollut Res Int. 2020 Sep.

Abstract

The current study was performed to evaluate the antiproliferative and apoptosis-inducing potential of n-hexane fraction from Cassia fistula L. (Caesalpinioideae) fruits. The antiproliferative property of the fraction was determined by MTT assay against cancer cell lines including HeLa, MG-63, IMR-32, and PC-3 with GI50 value of 97.69, 155.2, 143, and 160.2 μg/ml respectively. The fraction was further explored for its apoptotic effect using confocal, SEM, and flow cytometry studies in HeLa cells. It was observed that the treatment of fraction revealed fragmentation of DNA, chromatin condensation, membrane blebbing, and formation of apoptotic bodies in a dose-dependent manner. The fraction also showed a remarkable increase in the level of ROS, mitochondrial depolarization and G0/G1 phase cell cycle arrest, and induction in the phosphatidylserine externalization analyzed using Annexin V-FITC/PI double staining assay in HeLa cells. Kaempferol, Ellagic acid, and Epicatechin are the major phytoconstituents present in the fraction as revealed by the HPLC. The treatment of n-hexane fraction showed downregulation in the gene expression of Bcl-2 and upregulation in the expression level of p53, Bad, and caspase-3 genes analyzed using semi-quantitative RT-PCR in HeLa cells. These results suggest that n-hexane fraction from C. fistula inhibited the proliferation of cervical cancer cells efficiently by the induction of apoptosis. Graphical abstract.

Keywords: Antiproliferative; Apoptosis; Cassia fistula; HPLC; HeLa cells.

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References

    1. Abe K, Matsuki, N (2000) Measurement of cellular 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl- tetrazolium bromide (MTT) reduction activity and lactate dehydrogenase release using MTT. Neurosci Res 38:325–329.
    1. Baldivia DDS, Leite DF, Castro DTH, Campos JF, Santos UPD, Paredes-Gamero EJ, Carollo CA, Silva DB, de Picoli SK, Dos Santos EL (2018) Evaluation of in vitro antioxidant and anticancer properties of the aqueous extract from the stem bark of Stryphnodendron adstringens. Int J Mol Sci 19(8). https://doi.org/10.3390/ijms19082432
    1. Bhalerao SA, Kelkar TS (2012) Traditional medicinal uses, phytochemical profile and pharmacological activities of Cassia fistula Linn. Internet Res 1(5):79–84
    1. Bui NL, Pandey V, Zhu T, Ma L, Basappa LPE (2018) Bad phosphorylation as a target of inhibition in oncology. Cancer Lett 415:177–186. https://doi.org/10.1016/j.canlet.2017.11.017 - DOI
    1. Campbell KJ, Tait SWG (2018) Targeting BCL-2 regulated apoptosis in cancer. Open Biol 8:180002. https://doi.org/10.1098/rsob.180002 - DOI